Turning antagonists into allies: Bacterial-fungal interactions enhance the efficacy of controlling Fusarium wilt disease

IF 11.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Science Advances Pub Date : 2025-02-12 DOI:10.1126/sciadv.ads5089

Tuo Li, Xiaoteng Shi, Jiaguo Wang, Yihao Zhou, Tuokai Wang, Yan Xu, Zhihui Xu, Waseem Raza, Dongyang Liu, Qirong Shen

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Abstract

Intense microbial competition in soil has driven the evolution of resistance mechanisms, yet the implications of such evolution on plant health remain unclear. Our study explored the conversion from antagonism to coexistence between Bacillus velezensis ( Bv ) and Trichoderma guizhouense ( Tg ) and its effects on Fusarium wilt disease (FWD) control. We found a bacilysin transmembrane transporter ( Tg MFS4) in Tg , critical during cross-kingdom dialogue with B v. Deleting Tgmfs4 (Δ Tgmfs4 ) mitigated Bv - Tg antagonism, reduced bacilysin import into Tg , and elevated its level in the coculture environment. This increase acted as a feedback regulator, limiting overproduction and enhancing Bv biomass. Δ Tgmfs4 coinoculation with Bv demonstrated enhanced FWD control relative to wild-type Tg ( Tg -WT). In addition, the Tg -WT+ Bv consortium up-regulated antimycotic secretion pathways, whereas the Δ Tgmfs4 + Bv consortium enriched the CAZyme (carbohydrate-active enzyme) family gene expression in the rhizosphere, potentiating plant immune responses. This study elucidates the intricacies of bacterial-fungal interactions and their ramifications for plant health.

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化敌为友：细菌与真菌的相互作用提高了镰刀菌枯萎病的防治效果

土壤中激烈的微生物竞争推动了抗性机制的进化，但这种进化对植物健康的影响尚不清楚。本研究探讨了芽孢杆菌（Bacillus velezensis, Bv）与贵州木霉（Trichoderma guizhouense, Tg）从拮抗到共存的转化及其对枯萎病（Fusarium wilt disease， FWD）的防治效果。我们在Tg中发现了一个杆菌素跨膜转运蛋白（Tg MFS4），在与Bv的跨界对话中起关键作用。删除Tgmfs4 （Δ Tgmfs4）可以减轻Bv - Tg的拮抗作用，减少细菌素输入Tg，并提高其在共培养环境中的水平。这种增加起到了反馈调节器的作用，限制了生产过剩，提高了生物量。Δ Tgmfs4与Bv共接种显示出相对于野生型Tg （Tg -WT）增强的FWD控制。此外，Tg -WT+ Bv联合体上调了抗真菌分泌途径，而Δ Tgmfs4 + Bv联合体则增加了根际CAZyme（碳水化合物活性酶）家族基因的表达，增强了植物的免疫应答。本研究阐明了细菌-真菌相互作用的复杂性及其对植物健康的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Science Advances 综合性期刊-综合性期刊

CiteScore

21.40

自引率

1.50%

发文量

1937

审稿时长

29 weeks

期刊介绍： Science Advances, an open-access journal by AAAS, publishes impactful research in diverse scientific areas. It aims for fair, fast, and expert peer review, providing freely accessible research to readers. Led by distinguished scientists, the journal supports AAAS's mission by extending Science magazine's capacity to identify and promote significant advances. Evolving digital publishing technologies play a crucial role in advancing AAAS's global mission for science communication and benefitting humankind.