[Exploration of differences in decoction phase state, material form, and crystal form between Glycyrrhizae Radix et Rhizoma-Gypsum Fibrosum and Glycyrrhizae Radix et Rhizoma-CaSO_4·2H_2O based on supramolecules of traditional Chinese medicine].

Q3 Pharmacology, Toxicology and Pharmaceutics
Yao-Zhi Zhang, Wen-Min Pi, Xin-Ru Tan, Ran Xu, Xu Wang, Ming-Yang Xu, Xue-Mei Huang, Peng-Long Wang
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引用次数: 0

Abstract

With Glycyrrhizae Radix et Rhizoma-Gypsum Fibrosum drug pair as the research object, supramolecular chemistry of traditional Chinese medicine(TCM) was used to study differences between the compatibility of herbal medicine Glycyrrhizae Radix et Rhizoma with mineral medicine Gypsum Fibrosum and its main component CaSO_4·2H_2O, so as to preliminarily discuss the scientific connotation of compatibility of Gypsum Fibrosum in clinical application. A Malvern particle sizer, a scanning electron microscope(SEM), and a conductivity meter were used to observe and determine the physical properties such as microscopic morphology, particle size, and conductivity of Gypsum Fibrosum, CaSO_4·2H_2O, and water decoctions of them with Glycyrrhizae Radix et Rhizoma. An inductively coupled plasma optical emission spectrometer(ICP-OES) was employed to detect the inorganic metal elements in Glycyrrhizae Radix et Rhizoma-Gypsum Fibrosum and Glycyrrhizae Radix et Rhizoma-CaSO_4·2H_2O. Isothermal titration calorimetry(ITC) was conducted to quantify the interactions of Gypsum Fibrosum and CaSO_4·2H_2O with Glycyrrhizae Radix et Rhizoma. A Fourier transform infrared spectrometer(FTIR) was used to analyze the characteristic absorption peak change of Glycyrrhizae Radix et Rhizoma-Gypsum Fibrosum and Glycyrrhizae Radix et Rhizoma-CaSO_4·2H_2O. X-ray diffraction(XRD) was performed to determine the crystal structure and phase composition of Glycyrrhizae Radix et Rhizoma-Gypsum Fibrosum and Glycyrrhizae Radix et Rhizoma-CaSO_4·2H_2O. Further, glycyrrhizic acid(GA) was substituted for Glycyrrhizae Radix et Rhizoma to co-decoct with Gypsum Fibrosum, CaSO_4·2H_2O, and freeze-dried powder of their respective water decoctions. The results of XRD were used for verification analysis. The results showed that although CaSO_4·2H_2O is the main component of Gypsum Fibrosum, there were significant differences between their decoctions and between the decoctions of them with Glycyrrhizae Radix et Rhizoma. Specifically,(1) Both CaSO_4·2H_2O and Gypsum Fibrosum were amorphous fibrous. However, the particle size and conductivity were significantly different between the decoctions of CaSO_4·2H_2O and Gypsum Fibrosum alone.(2) Under SEM, Glycyrrhizae Radix et Rhizoma-CaSO_4·2H_2O was a hybrid system with various morphologies, while Glycyrrhizae Radix et Rhizoma-Gypsum Fibrosum presented uniform nanoparticles.(3) The particle sizes and conductivities of Glycyrrhizae Radix et Rhizoma-CaSO_4·2H_2O and Glycyrrhizae Radix et Rhizoma-Gypsum Fibrosum were significantly different and did not follow the same tendency as those of the decoctions of CaSO_4·2H_2O and Gypsum Fibrosum alone.(4) Compared with Glycyrrhizae Radix et Rhizoma-CaSO_4·2H_2O, Glycyrrhizae Radix et Rhizoma-Gypsum Fibrosum had stronger molecular binding ability and functional group structure change.(5) The crystal form was largely different between the freeze-dried powder of CaSO_4·2H_2O decoction and Gypsum Fibrosum decoction, and their crystal forms were also significantly different from those of the freeze-dried powder of Glycyrrhizae Radix et Rhizoma-CaSO_4·2H_2O and Glycyrrhizae Radix et Rhizoma-Gypsum Fibrosum decoctions. The reason for the series of differences is that Gypsum Fibrosum is richer in trace elements than CaSO_4·2H_2O. The XRD results of GA-Gypsum Fibrosum and GA-CaSO_4·2H_2O decoctions further prove the importance of trace elements in Gypsum Fibrosum for supramolecule formation. This research preliminarily reveals the influence of compatibility of Gypsum Fibrosum or CaSO_4·2H_2O on decoction phase state, material form, and crystal form, providing a basis for the rational clinical application of Gypsum Fibrosum.

[基于中药超分子探讨甘草-石膏纤维与甘草- caso_4·2H_2O煎剂相态、物质形态、结晶形态的差异]。
以甘草-石膏纤维药物对为研究对象,运用中医超分子化学方法,研究中药甘草与矿物药石膏纤维及其主要成分CaSO_4·2H_2O配伍的差异,初步探讨石膏纤维配伍在临床应用中的科学内涵。采用Malvern粒度仪、扫描电镜(SEM)和电导率仪对石膏纤维、CaSO_4·2H_2O及其与甘草水煎液的微观形貌、粒径和电导率等物理性质进行了观察和测定。采用电感耦合等离子体发射光谱仪(ICP-OES)对甘草-石膏纤维和甘草- caso_4·2H_2O中的无机金属元素进行了检测。采用等温滴定量热法(ITC)测定了石膏纤维和CaSO_4·2H_2O与甘草的相互作用。采用傅里叶变换红外光谱仪(FTIR)分析了甘草-石膏纤维和甘草- caso_4·2H_2O的特征吸收峰变化。采用x射线衍射(XRD)测定了甘草-石膏纤维和甘草- caso_4·2H_2O的晶体结构和物相组成。以甘草酸(GA)代替甘草酸,与石膏纤维、CaSO_4·2H_2O、冻干粉等水煎液共煎。采用XRD结果进行验证分析。结果表明,虽然CaSO_4·2H_2O是石膏纤维的主要成分,但其煎液及与甘草煎液的含量差异显著。具体而言,(1)CaSO_4·2H_2O和石膏纤维均为无定形纤维。(2)扫描电镜下,甘草与CaSO_4·2H_2O是一个具有多种形态的杂化体系;(3)甘草-CaSO_4·2H_2O和甘草-石膏-纤维的颗粒大小和电导率存在显著差异,且与单独使用CaSO_4·2H_2O和石膏纤维的颗粒大小和电导率的变化趋势不一致。(5) CaSO_4·2H_2O煎剂冻干粉和石膏纤维煎剂的结晶形态差异较大,与CaSO_4·2H_2O煎剂冻干粉和甘草-石膏纤维煎剂的结晶形态也有显著差异。造成这一系列差异的原因是石膏纤维比CaSO_4·2H_2O含有更丰富的微量元素。ga -石膏纤维和GA-CaSO_4·2H_2O煎剂的XRD结果进一步证明了石膏纤维中微量元素对超分子形成的重要性。本研究初步揭示了石膏与CaSO_4·2H_2O的配伍对煎剂相态、物质形态、结晶形态的影响,为石膏的合理临床应用提供依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Zhongguo Zhongyao Zazhi
Zhongguo Zhongyao Zazhi Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)
CiteScore
1.50
自引率
0.00%
发文量
581
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