Bifidobacterium longum NSP001-derived extracellular vesicles ameliorate ulcerative colitis by modulating T cell responses in gut microbiota-(in)dependent manners.

IF 7.8 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

npj Biofilms and Microbiomes Pub Date : 2025-02-10 DOI:10.1038/s41522-025-00663-4

Xinke Nie, Qiqiong Li, Haihua Ji, Shanshan Zhang, Yuchen Wang, Junhua Xie, Shaoping Nie

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引用次数: 0

Abstract

Recent studies have shown that intestinal commensal bacteria-derived vesicles may have potential effects in alleviating ulcerative colitis (UC). Although Bifidobacterium longum is widely used to prevent colitis, the potential role of B. longum-derived extracellular vesicles has yet to be explored. Here, we extracted B. longum NSP001-derived extracellular vesicles (NEVs) and investigated the regulatory roles of NEVs in colitis. Our results demonstrated that NEVs alleviate UC by improving intestinal barrier, modulating immune cell differentiation, and promoting the production of SCFAs. NEVs' improvement of inflammation in pseudo-germ-free mice implies that the anti-inflammatory effect of NEVs does not exclusively depend on the regulation of gut microbiota. In conclusion, we suggest that B. longum NSP001 improves UC through the secretion of NEVs. In addition, the study emphasizes the critical role of NEVs in maintaining host immune homeostasis via suppressing STAT3 pathway, thereby highlighting their potential as a novel postbiotic to alleviate UC.

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长双歧杆菌nsp001衍生的细胞外囊泡通过调节肠道微生物群依赖方式的T细胞反应来改善溃疡性结肠炎。

最近的研究表明，肠道共生菌衍生的囊泡可能对缓解溃疡性结肠炎（UC）有潜在的作用。虽然长双歧杆菌被广泛用于预防结肠炎，但长双歧杆菌衍生的细胞外囊泡的潜在作用尚未被探索。在这里，我们提取了长芽孢杆菌nsp001衍生的细胞外囊泡（nev），并研究了nev在结肠炎中的调节作用。我们的研究结果表明，nev通过改善肠道屏障、调节免疫细胞分化和促进scfa的产生来缓解UC。新能源汽车对伪无菌小鼠炎症的改善表明，新能源汽车的抗炎作用并不完全依赖于肠道微生物群的调节。综上所述，我们认为长芽孢杆菌NSP001通过分泌nev来改善UC。此外，该研究强调了nev通过抑制STAT3通路在维持宿主免疫稳态方面的关键作用，从而强调了它们作为一种新型后生物缓解UC的潜力。

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来源期刊

npj Biofilms and Microbiomes Immunology and Microbiology-Microbiology

CiteScore

12.10

自引率

3.30%

发文量

审稿时长

9 weeks

期刊介绍： npj Biofilms and Microbiomes is a comprehensive platform that promotes research on biofilms and microbiomes across various scientific disciplines. The journal facilitates cross-disciplinary discussions to enhance our understanding of the biology, ecology, and communal functions of biofilms, populations, and communities. It also focuses on applications in the medical, environmental, and engineering domains. The scope of the journal encompasses all aspects of the field, ranging from cell-cell communication and single cell interactions to the microbiomes of humans, animals, plants, and natural and built environments. The journal also welcomes research on the virome, phageome, mycome, and fungome. It publishes both applied science and theoretical work. As an open access and interdisciplinary journal, its primary goal is to publish significant scientific advancements in microbial biofilms and microbiomes. The journal enables discussions that span multiple disciplines and contributes to our understanding of the social behavior of microbial biofilm populations and communities, and their impact on life, human health, and the environment.