Hypoxia Impairs Migration of Human Dermal Lymph Endothelial Cells: Implications for Lymphedema Pathogenesis.

IF 1.9 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Lymphatic research and biology Pub Date : 2025-06-01 Epub Date: 2025-02-10 DOI:10.1089/lrb.2024.0019
Chihiro Matsui, Hiroshi Koide, Hatan Mortada, Reiko Tsukuura, Toko Miyazaki, Hayahito Sakai, Hiroshi Mizuno, Takumi Yamamoto
{"title":"Hypoxia Impairs Migration of Human Dermal Lymph Endothelial Cells: Implications for Lymphedema Pathogenesis.","authors":"Chihiro Matsui, Hiroshi Koide, Hatan Mortada, Reiko Tsukuura, Toko Miyazaki, Hayahito Sakai, Hiroshi Mizuno, Takumi Yamamoto","doi":"10.1089/lrb.2024.0019","DOIUrl":null,"url":null,"abstract":"<p><p><b><i>Introduction:</i></b> Lymphedema, characterized by abnormal lymph circulation, is a chronic debilitating condition often associated with chronic inflammation and hypoxia in lymphedematous tissue. Previously, using videocapillaroscopy, we found that the severity of lymphedema damages vasa vasorum vessels on the surface of lymph vessels, significantly compromising the oxygen supply environment to lymph endothelial cells. While previous research has indicated the inhibitory effects of hypoxic conditions on the proliferation of human dermal lymph endothelial cells (HDLECs), the impact on HDLEC migration remains unclear. This study aims to investigate the migration ability of HDLECs under hypoxic conditions, shedding light on the potential mechanisms underlying lymphedema progression and offering insights into therapeutic strategies. <b><i>Materials and Methods and Results:</i></b> Purchased HDLECs were cultured under normoxic (2% O<sub>2</sub>) and hypoxic (1% O<sub>2</sub>) conditions. Migration assays were performed using a scratch assay to assess the migratory capabilities of HDLECs. The experiment monitored cell migration over a period of 6 hours. Experiments were performed in triplicate. HDLECs were cultured to full confluence before the scratch assay. The results revealed a significant reduction in HDLEC migration under hypoxic conditions compared to normoxic conditions after 6 hours (<i>p</i> < 0.001). This suggests that hypoxic environments directly impair HDLEC migration. <b><i>Conclusion:</i></b> Hypoxia negatively affects HDLEC migration, potentially exacerbating lymphedema. Protecting vasa vasorum may preserve HDLEC migration and lymphangiogenesis. Further exploration of hypoxia-HDLEC interactions is crucial for understanding lymphedema pathogenesis and developing therapies.</p>","PeriodicalId":18168,"journal":{"name":"Lymphatic research and biology","volume":" ","pages":"184-187"},"PeriodicalIF":1.9000,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Lymphatic research and biology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1089/lrb.2024.0019","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/2/10 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}
引用次数: 0

Abstract

Introduction: Lymphedema, characterized by abnormal lymph circulation, is a chronic debilitating condition often associated with chronic inflammation and hypoxia in lymphedematous tissue. Previously, using videocapillaroscopy, we found that the severity of lymphedema damages vasa vasorum vessels on the surface of lymph vessels, significantly compromising the oxygen supply environment to lymph endothelial cells. While previous research has indicated the inhibitory effects of hypoxic conditions on the proliferation of human dermal lymph endothelial cells (HDLECs), the impact on HDLEC migration remains unclear. This study aims to investigate the migration ability of HDLECs under hypoxic conditions, shedding light on the potential mechanisms underlying lymphedema progression and offering insights into therapeutic strategies. Materials and Methods and Results: Purchased HDLECs were cultured under normoxic (2% O2) and hypoxic (1% O2) conditions. Migration assays were performed using a scratch assay to assess the migratory capabilities of HDLECs. The experiment monitored cell migration over a period of 6 hours. Experiments were performed in triplicate. HDLECs were cultured to full confluence before the scratch assay. The results revealed a significant reduction in HDLEC migration under hypoxic conditions compared to normoxic conditions after 6 hours (p < 0.001). This suggests that hypoxic environments directly impair HDLEC migration. Conclusion: Hypoxia negatively affects HDLEC migration, potentially exacerbating lymphedema. Protecting vasa vasorum may preserve HDLEC migration and lymphangiogenesis. Further exploration of hypoxia-HDLEC interactions is crucial for understanding lymphedema pathogenesis and developing therapies.

缺氧损害人真皮淋巴内皮细胞的迁移:对淋巴水肿发病的影响。
淋巴水肿以淋巴循环异常为特征,是一种慢性衰弱性疾病,常与淋巴水肿组织的慢性炎症和缺氧有关。之前,我们通过视频毛细血管镜检查发现,淋巴水肿的严重程度会损害淋巴血管表面的血管血管,严重影响淋巴内皮细胞的供氧环境。虽然先前的研究表明缺氧条件对人真皮淋巴内皮细胞(HDLECs)的增殖有抑制作用,但对HDLEC迁移的影响尚不清楚。本研究旨在探讨HDLECs在缺氧条件下的迁移能力,揭示淋巴水肿进展的潜在机制,并为治疗策略提供见解。材料、方法和结果:购买的HDLECs在常氧(2% O2)和缺氧(1% O2)条件下培养。采用划痕法进行迁移试验,以评估HDLECs的迁移能力。该实验在6小时内监测细胞迁移。实验一式三次。在划痕实验前,将HDLECs培养至充分融合。结果显示,与常氧条件相比,低氧条件下HDLEC迁移在6小时后显著减少(p < 0.001)。这表明缺氧环境直接影响HDLEC的迁移。结论:缺氧对HDLEC迁移有负面影响,可能加剧淋巴水肿。保护血管可以保护HDLEC的迁移和淋巴管生成。进一步探索缺氧- hdlec相互作用对于了解淋巴水肿的发病机制和开发治疗方法至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Lymphatic research and biology
Lymphatic research and biology Medicine-Cardiology and Cardiovascular Medicine
CiteScore
3.10
自引率
7.10%
发文量
85
审稿时长
>12 weeks
期刊介绍: Lymphatic Research and Biology delivers the most current peer-reviewed advances and developments in lymphatic biology and pathology from the world’s leading biomedical investigators. The Journal provides original research from a broad range of investigative disciplines, including genetics, biochemistry and biophysics, cellular and molecular biology, physiology and pharmacology, anatomy, developmental biology, and pathology. Lymphatic Research and Biology coverage includes: -Vasculogenesis and angiogenesis -Genetics of lymphatic disorders -Human lymphatic disease, including lymphatic insufficiency and associated vascular anomalies -Physiology of intestinal fluid and protein balance -Immunosurveillance and immune cell trafficking -Tumor biology and metastasis -Pharmacology -Lymphatic imaging -Endothelial and smooth muscle cell biology -Inflammation, infection, and autoimmune disease
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信