Involvement of Nerve Growth Factor in the Reparative Reaction to Osteonecrotic Lesions

IF 2.1 3区 医学 Q2 ORTHOPEDICS
Yusuke Ayabe, Goro Motomura, Ryosuke Yamaguchi, Takeshi Utsunomiya, Hidenao Tanaka, Kosei Sakamoto, Yasuharu Nakashima
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引用次数: 0

Abstract

The reparative zone in femoral head osteonecrosis is pivotal for repair; however, the repair response mechanism remains poorly understood. Although neurotrophic nerve growth factor significantly contributes to bone formation in fatigue fractures and ectopic ossification, the relationship between nerve growth factor and the repair response to osteonecrosis remains unexplored. We aimed to elucidate the role of nerve growth factor in osteonecrosis repair. Twenty-seven wholly resected femoral heads diagnosed with Japanese Investigation Committee Stage 3 femoral head osteonecrosis, excluding those with severe collapse, were analyzed. Histopathological diagnosis confirmed the presence of necrotic, reparative, and viable zones in all examined femoral heads. Quantitative evaluation of immunohistological staining, including nerve growth factor, vascular endothelial growth factor, osteocalcin, CD31, and TUBB3, was conducted in each zone. Additionally, micro-computed tomography was used to measure the trabecular bone microstructure in the reparative zone. Nerve growth factor expression was detected in all 27 femoral heads with osteonecrosis, exhibiting a significantly higher prevalence in the reparative zone than in other regions (p < 0.0001). Nerve growth factor was predominantly distributed on the necrotic side within the reparative zone, rather than the viable side. In the reparative zone, nerve growth factor expression was positively correlated with bone formation parameters derived from micro-computed tomography images. Vascular endothelial growth factor, osteocalcin, CD31(+) vascular endothelial cells, and TUBB3(+) nerve cells also significantly increased in the reparative zone. In conclusion, nerve growth factor expression was consistent across all femoral heads with osteonecrosis and may play a role in reparative reaction to osteonecrotic lesions.

神经生长因子在骨坏死损伤修复反应中的作用。
股骨头坏死的修复区是修复的关键;然而,修复反应机制仍然知之甚少。尽管神经营养性神经生长因子对疲劳骨折和异位骨化的骨形成有重要作用,但神经生长因子与骨坏死修复反应的关系仍未得到充分研究。我们旨在阐明神经生长因子在骨坏死修复中的作用。分析27例全切除股骨头诊断为日本调查委员会第3期股骨头坏死,不包括严重塌陷的股骨头。组织病理学诊断证实在所有检查的股骨头中存在坏死区、修复区和活区。定量评价各区免疫组织染色,包括神经生长因子、血管内皮生长因子、骨钙素、CD31、TUBB3。此外,显微计算机断层扫描用于测量修复区骨小梁的微观结构。神经生长因子在27例股骨头坏死患者中均有表达,在修复区明显高于其他区域
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Orthopaedic Research®
Journal of Orthopaedic Research® 医学-整形外科
CiteScore
6.10
自引率
3.60%
发文量
261
审稿时长
3-6 weeks
期刊介绍: The Journal of Orthopaedic Research is the forum for the rapid publication of high quality reports of new information on the full spectrum of orthopaedic research, including life sciences, engineering, translational, and clinical studies.
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