{"title":"Prevalence and molecular characterization of <i>Enterocytozoon bieneusi</i> in endangered Eld's deer (<i>Rucervus eldii</i>) in Hainan, China.","authors":"Yun Zhang, Guangxu Ren, Qingqing Lu, Jiaqi Li, Yu Qiang, Youyou Li, Xiuyi Lai, Yuan Wang, Xingyue Yu, Sheng Lei, Yu Li, Yunxing Chang, Xianrong Liu, Xuning Qi, Zhi Xie, Tingting Li, Jiang Du, Rui Duan, Xinyu Chang, Hesheng Wang, Gang Lu","doi":"10.3389/fvets.2025.1521055","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong><i>Enterocytozoon bieneusi</i> is one of the most frequent microsporidia species causing digestive disorder mainly diarrhea in humans and animals. Eld's deer (<i>Rucervus eldii</i>) is the class I national key protected wildlife and only distributed on Hainan Island in China. No report on the prevalence and molecular characterization of <i>E. bieneusi</i> in wild Eld's deer worldwide.</p><p><strong>Methods: </strong>217 fecal samples were collected from Eld's deer in two isolated habitats of a nature reserve in Hainan, and examined by nested Polymerase Chain Reaction (PCR) targeting the internal transcribed spacer (ITS) region.</p><p><strong>Results and discussion: </strong>The overall prevalence of <i>E. bieneusi</i> in Eld's deer was 17.5% (38/217), with 13.5% (12/89) and 20.3% (26/128) in habitats 1 and 2, respectively. Seven ITS genotypes were identified, including five known genotypes: D (<i>n</i> = 19), Peru11 (<i>n</i> = 10), EbpC (<i>n</i> = 5), Peru8 (<i>n</i> = 1) and Type IV (<i>n</i> = 1), and two novel genotypes: HNED-I and HNED-II (one each). Genotypes Peru8 and Peru11 were firstly identified in cervids. Phylogenetic analysis showed that all the detected genotypes belonged to zoonotic Group 1. The results implied that the further research on threaten of <i>E. bieneusi</i> to endangered Eld's deer and potential risks for public health is necessary.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1521055"},"PeriodicalIF":2.6000,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11808153/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Veterinary Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.3389/fvets.2025.1521055","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Introduction: Enterocytozoon bieneusi is one of the most frequent microsporidia species causing digestive disorder mainly diarrhea in humans and animals. Eld's deer (Rucervus eldii) is the class I national key protected wildlife and only distributed on Hainan Island in China. No report on the prevalence and molecular characterization of E. bieneusi in wild Eld's deer worldwide.
Methods: 217 fecal samples were collected from Eld's deer in two isolated habitats of a nature reserve in Hainan, and examined by nested Polymerase Chain Reaction (PCR) targeting the internal transcribed spacer (ITS) region.
Results and discussion: The overall prevalence of E. bieneusi in Eld's deer was 17.5% (38/217), with 13.5% (12/89) and 20.3% (26/128) in habitats 1 and 2, respectively. Seven ITS genotypes were identified, including five known genotypes: D (n = 19), Peru11 (n = 10), EbpC (n = 5), Peru8 (n = 1) and Type IV (n = 1), and two novel genotypes: HNED-I and HNED-II (one each). Genotypes Peru8 and Peru11 were firstly identified in cervids. Phylogenetic analysis showed that all the detected genotypes belonged to zoonotic Group 1. The results implied that the further research on threaten of E. bieneusi to endangered Eld's deer and potential risks for public health is necessary.
期刊介绍：
Frontiers in Veterinary Science is a global, peer-reviewed, Open Access journal that bridges animal and human health, brings a comparative approach to medical and surgical challenges, and advances innovative biotechnology and therapy.
Veterinary research today is interdisciplinary, collaborative, and socially relevant, transforming how we understand and investigate animal health and disease. Fundamental research in emerging infectious diseases, predictive genomics, stem cell therapy, and translational modelling is grounded within the integrative social context of public and environmental health, wildlife conservation, novel biomarkers, societal well-being, and cutting-edge clinical practice and specialization. Frontiers in Veterinary Science brings a 21st-century approach—networked, collaborative, and Open Access—to communicate this progress and innovation to both the specialist and to the wider audience of readers in the field.
Frontiers in Veterinary Science publishes articles on outstanding discoveries across a wide spectrum of translational, foundational, and clinical research. The journal''s mission is to bring all relevant veterinary sciences together on a single platform with the goal of improving animal and human health.
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