Generation of cross-reactive DNA aptamers to construct the fluorescent sensing array for identifying the origin of Toad Venom.

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2025-02-09 DOI:10.1016/j.talanta.2025.127705

Jiwei Li, Guocai Liu, Haixiang Yu, Hongyue Ma, Xiufeng Liu, Jiangwei Tian, Boyang Yu

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引用次数: 0

Abstract

Toad venom, a traditional Chinese medicine, has long been used to treat various challenging ailments. Its effectiveness and toxicity can vary depending on the types and concentrations of bufadienolides, which vary from region to region. However, identifying the origin of toad venom is challenging due to the absence of distinct visual characteristics of the original animals. Therefore, developing a scientific and practical method for origin identification is crucial to ensure the safety and efficacy of toad venom. Integrating a fluorescent sensing array with cross-reactive aptamers provides a promising solution to this issue. We isolated cross-reactive aptamers using a combination of complex target-directed SELEX and convergent selection strategies. During the selection process, we used an immobilized stem-loop library to select aptamers and evaluated the enrichment rate and pool affinity using gel elution assays. After high-throughput sequencing, we selected three cross-reactive aptamers designated N4.8, N2.4, and S1 that exhibit distinct binding profiles for bufadienolides as the biorecognition elements for the fluorescent sensing array. This sensor is capable of distinguishing toad venom from different origins with high accuracy (98.7 %), offering convenient operation, and providing a new method for detecting the origin of toad venom.

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.