Antibiofilm efficiency of silver and copper nanoparticle incorporated calcium hydroxide as an intracanal medicament: An in vitro study

Q1 Medicine

Journal of oral biology and craniofacial research Pub Date : 2025-02-11 DOI:10.1016/j.jobcr.2025.01.020

Murali H. Rao , Krishnan Rajkumar , Gopal Pavithra , Ramya Ramadoss , Suryanarayanan Shankar , Krishnamachari Janani , Kumar Chandan Srivastava , Deepti Shrivastava , Dinesh Rokaya

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引用次数: 0

Abstract

Background and objectives

Silver (Ag) and Copper (Cu) Nanoparticles (NP) are a potential substitute for disinfection during endodontic therapy because of their antibacterial action against an array of pathogens, including resistant strains. This study aimed to assess the antibiofilm efficiency of calcium hydroxide modified with Ag and Cu NP suspension against E. faecalis, using confocal laser scanning microscopy (CLSM) and colony forming units (CFU) analysis.

Methods

Dentine blocks were inoculated with Enterococcus faecalis for one week and 1 week-old biofilm of E. feacalis were randomly divided into 4 groups, control, calcium hydroxide, calcium hydroxide +2 % Cu NP and calcium hydroxide +2 % Ag NP. Following the incubation of the specimens with the medicament for 24 h at 37C, CLSM was used to evaluate the reduction in biovolume of the biofilm and the CFU was determined to assess the antimicrobial action of the NP-modified calcium hydroxide.

Results

CLSM images revealed a significant reduction in the thickness of biofilm in both Ag and Cu groups compared to calcium hydroxide alone. The CFU results showed that group 4 and group 3 showed significantly less CFU followed by group 2 and group 1 (p < 0.001).

Conclusion

Adding Cu and Ag NP to calcium hydroxide intracanal medication significantly increases its antibacterial activity against E. faecalis.

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来源期刊

Journal of oral biology and craniofacial research Medicine-Otorhinolaryngology

CiteScore

4.90

自引率

0.00%

发文量

133

审稿时长

167 days

期刊介绍： Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.