BeyondⓇ extender in donkey semen: evaluation at 5 °C and 17 °C over 7 days

IF 1.3 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science Pub Date : 2025-02-01 DOI:10.1016/j.jevs.2024.105270

M. Caldevilla, D. Neild, A. Ferrante, I. Cáceres Monie, C. Arraztoa, J. Plaza, J. Otero, M. Gambarotta, M. Miragaya

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引用次数: 0

Abstract

The objective of this study was to evaluate the ability of the equine commercial extender Beyond^Ⓡ, both alone and with the addition of fresh egg-yolk (EY), to maintain donkey sperm motility, viability and acrosomes at 5 °C and 17 °C over 7 days or until progressive motility (PM) was lower than 20%. Fifteen ejaculates from 5 fertile jacks were diluted in EquiPlusTM, incubated 30 minutes at room temperature, centrifuged and resuspended in: Beyond^Ⓡ(B), B with 2% EY (BY) and skim milk-based extender with 2% EY (KY). Sperm kinematic parameters (CASA; AndroVision^Ⓡ) and viability and acrosome integrity (FITC-PNA/PI using flow cytometry; BD FACSCanto II^Ⓡ) were evaluated daily. Either ANOVA or Kruskal Wallis was used to analyze the data and P<0.05 was considered significant. In the samples at 5 °C a significant decrease in % PM was observed between day 7 (B: 34.18±8.0; BY: 36.14 ±17.9; KY: 30.08±0) compared to 24h (B: 50.04±20.8; BY: 59.19±20.4; KY: 63.29±17.1). At 72h significantly higher percentages of total motility (TM) were observed for B and BY compared to KY. PM in B was not significantly different to BY and KY at each evaluation time. However, the number of samples with PM > 20% at day 7 were greater in the BY samples (9/15) compared to B (5/15) and KY (1/15). Additionally, a significant decrease in live acrosome intact (LAI) sperm was observed on day 7 (B: 19.55±15.63; BY: 9.99±10.03; KY: 36.79±0) compared to 24h (B: 43.24±19.18; BY: 55.56±16.96; KY: 61.89±12.26). No significant differences were observed in % LAI for B compared to BY and KY at each evaluation time. At 17 °C, a significant decrease in percentage PM was observed on day 7 (B: 33.5±11.55; BY: 36.69±8.6) compared to 24h (B: 60.47±15.1; BY: 65.57±13.1). TM and PM in B were not significantly different to BY at each evaluation time. The number of samples with PM > 20% at day 7 was greater in the BY samples (9/15) compared to B (3/15). Additionally, % LAI was significantly lower on day 7 (B: 26.67±28.82; BY: 14.49±14.65) compared to 24h (B: 45.40±28.68; BY: 48.39±28.83). No significant differences were observed in % LAI for B compared to BY at each evaluation time. No significant differences were observed in any of the sperm parameters between both temperatures (5 and 17 °C) for any of the extenders assayed. To conclude, although Beyond^Ⓡ can be used to preserve donkey semen for 7 days, both at 5 and 17 °C, it is noteworthy that many more samples lasted 7 days with PM > 20% when extended in Beyond^Ⓡ with 2% egg-yolk.

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驴精液中的Ⓡ扩展剂：在5°C和17°C下7天的评估

本研究的目的是评估马商业扩展剂BeyondⓇ在单独使用和添加新鲜蛋黄（EY）的情况下，在5°C和17°C条件下维持驴精子活力、活力和顶体7天或直到进行性活力（PM）低于20%的能力。用EquiPlusTM稀释5头可育千牛的15次射精，室温孵育30分钟，离心后重悬于：BeyondⓇ(B)， B含2% EY （BY）和脱脂乳基填充剂含2% EY （KY）。精子运动参数；AndroVisionⓇ)和顶体完整性（流式细胞术检测FITC-PNA/PI）；BD FACSCanto IIⓇ)每日进行评估。采用方差分析（ANOVA）或Kruskal Wallis对数据进行分析，P<0.05 bb0。在5°C的样品中，在第7天之间观察到% PM显著下降(B: 34.18±8.0；比：36.14±17.9；KY: 30.08±0)与24h相比(B: 50.04±20.8；: 59.19±20.4;肯塔基州:63.29±17.1)。72h时，与KY相比，B和BY的总运动率（TM）显著提高。各评价时段B组PM与BY和KY组PM差异不显著。然而，PM >；第7天，与B（5/15）和KY（1/15）相比，BY样品（9/15）的20%更高。此外，在第7天观察到活顶体完整精子（LAI）显著减少(B: 19.55±15.63；: 9.99±10.03;KY: 36.79±0)与24h相比(B: 43.24±19.18；: 55.56±16.96;肯塔基州:61.89±12.26)。在每个评估时间，与BY和KY相比，B的LAI %没有显著差异。在17°C时，第7天PM百分比显著下降(B: 33.5±11.55；BY: 36.69±8.6)与24h相比(B: 60.47±15.1；: 65.57±13.1)。B组的TM和PM在各评价时间与BY无显著差异。含PM >的样本数；第7天，BY样品（9/15）的20%高于B样品（3/15）。此外，LAI %在第7天显著降低(B: 26.67±28.82；BY: 14.49±14.65)与24h相比(B: 45.40±28.68；: 48.39±28.83)。在每个评估时间，与BY相比，B的LAI %没有显著差异。在两种温度（5°C和17°C）之间，对任何一种扩增剂的精子参数均未观察到显著差异。综上所述，尽管BeyondⓇ可以在5°C和17°C下保存驴精液7天，但值得注意的是，在PM >；在BeyondⓇ中加入2%的蛋黄，达到20%。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Equine Veterinary Science 农林科学-兽医学

CiteScore

2.70

自引率

7.70%

发文量

249

审稿时长

77 days

期刊介绍： Journal of Equine Veterinary Science (JEVS) is an international publication designed for the practicing equine veterinarian, equine researcher, and other equine health care specialist. Published monthly, each issue of JEVS includes original research, reviews, case reports, short communications, and clinical techniques from leaders in the equine veterinary field, covering such topics as laminitis, reproduction, infectious disease, parasitology, behavior, podology, internal medicine, surgery and nutrition.