Metasurface Plasmon Resonance Biosensor Enhanced with Dual Gold Nanoparticles for the Ultrasensitive Quantitative Detection of C-Reactive Protein

IF 9.1 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Letters Pub Date : 2025-01-28 DOI:10.1021/acs.nanolett.4c0604910.1021/acs.nanolett.4c06049

Youqian Chen, Yihui Yang, Hanlin Zhou, Wenshuai Yao, Shaoqi Zeng, Mingqian Chen, Wenjun Hu, Gang Logan Liu and Liping Huang*,

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Abstract

The pursuit of cutting-edge diagnostic systems capable of detecting biomarkers with exceptional sensitivity and precision is crucial for the timely and accurate monitoring of inflammatory responses. In this study, we introduce a dual gold nanoparticle-enhanced metasurface plasmon resonance (Bi-MSPR) biosensor for the ultrasensitive detection of C-reactive protein (CRP). The Bi-MSPR sensor is constructed upon a nanocup array chip with gradient-free electron density, where an innovative metasurface structure is built using a PEI-immobilized dual-gold nanoparticle amplification system. This strategy greatly amplifies the local free electric field, resulting in a signal enhancement exceeding 10-fold, with a detection limit of 0.04 ng/mL for CRP in buffer solutions and 0.017 μg/mL for serum samples. Clinical validation demonstrates a 95.83% detection rate with no cross-reactivity, highlighting the sensor’s remarkable specificity and sensitivity for CRP detection. The Bi-MSPR biosensor thus represents a rapid, sensitive, and specific diagnostic tool for CRP detection.

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双金纳米粒子增强超表面等离子共振生物传感器用于c反应蛋白的超灵敏定量检测

追求能够检测生物标志物的尖端诊断系统，具有卓越的灵敏度和精度，对于及时准确地监测炎症反应至关重要。在这项研究中，我们引入了一种双金纳米粒子增强的超表面等离子体共振（Bi-MSPR）生物传感器，用于超灵敏检测c反应蛋白（CRP）。Bi-MSPR传感器构建在无梯度电子密度的纳米杯阵列芯片上，其中采用pei固定化双金纳米粒子扩增系统构建了创新的超表面结构。该策略极大地放大了局部自由电场，使信号增强超过10倍，缓冲液中CRP的检出限为0.04 ng/mL，血清样品的检出限为0.017 μg/mL。临床验证，该传感器的检出率为95.83%，无交叉反应，对CRP检测具有显著的特异性和敏感性。因此，Bi-MSPR生物传感器代表了一种快速、敏感和特异性的CRP检测诊断工具。

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来源期刊

Nano Letters 工程技术-材料科学：综合

CiteScore

16.80

自引率

2.80%

发文量

1182

审稿时长

1.4 months

期刊介绍： Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including: - Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale - Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies - Modeling and simulation of synthetic, assembly, and interaction processes - Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance - Applications of nanoscale materials in living and environmental systems Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.