Differential antibiosis predisposes mice to Campylobacter jejuni infection: Deeper insights into the impact of the gut microbiota composition in colonization resistance.

European journal of microbiology & immunology Pub Date : 2025-02-10 Print Date: 2025-03-19 DOI:10.1556/1886.2024.00140
Nizar W Shayya, Soraya Mousavi, Kerstin Stingl, Stefan Bereswill, Markus M Heimesaat
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Abstract

Conventional laboratory mice are protected from oral Campylobacter jejuni infection due to colonization resistance (CR) mediated by their host-specific gut microbiota. Here, we used differential effects of distinct antibiotics on gut microbiota composition to identify microbial groups associated with CR against C. jejuni. Therefore, specific pathogen-free (SPF) mice were subjected to ampicillin plus sulbactam (A/S), ciprofloxacin (CIP), or vancomycin (VAN) via the drinking water for 28 days or left untreated before peroral C. jejuni challenge. Cultural analyses revealed that CR displayed by untreated mice was abrogated by A/S treatment, but only reduced in mice treated with CIP or VAN. Notably, differential analysis of antibiotic-induced microbiota changes and C. jejuni colonization dynamics identified lactobacilli and Clostridium leptum as key microbial groups that were associated with CR. Notably, the complete eradication of intestinal bacteria in A/S treated mice supported high intestinal C. jejuni colonization levels which triggered apoptosis and inflammatory responses accompanied by enhanced expression of matrix-degrading gelatinases in the colon. In conclusion, A/S treated mice represent a valuable infection model for the study of campylobacteriosis and the treatment of mice with specific antibiotics support the investigation of molecular mechanisms involved in CR against enteropathogens.

不同抗生素使小鼠易受空肠弯曲杆菌感染:深入了解肠道微生物群组成对定植抗性的影响。
由于宿主特异性肠道菌群介导的定植抗性(CR),传统实验室小鼠免受口腔空肠弯曲杆菌感染。在这里,我们使用不同抗生素对肠道菌群组成的差异影响来确定与CR对抗空肠梭菌相关的微生物群。因此,特定无病原体(SPF)小鼠在经口空肠梭菌攻毒前,通过饮用水给予氨苄西林加舒巴坦(A/S)、环丙沙星(CIP)或万古霉素(VAN) 28天或不给药。培养分析显示,A/S处理消除了未处理小鼠的CR,而CIP或VAN处理仅降低了CR。值得注意的是,抗生素诱导的微生物群变化和空肠梭菌定植动态的差异分析表明,乳酸菌和瘦梭菌是与CR相关的关键微生物群。值得注意的是,A/S处理小鼠肠道细菌的完全根除支持高肠道空肠梭菌定植水平,从而引发细胞凋亡和炎症反应,同时增强结肠中基质降解明胶酶的表达。综上所述,A/S处理小鼠为研究弯曲杆菌病提供了一种有价值的感染模型,而用特异性抗生素治疗小鼠有助于研究CR对抗肠道病原体的分子机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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