Hironobu Naiki, Mona Johnson, Kaylee Walters, Alexander Carpinteiro, M Teresa Cibeira, Anita D'Souza, Efstathios Kastritis, Mathew S Maurer, Lisa Mendelson, Giampaolo Merlini, Eli Muchtar, Giovanni Palladini, Eloisa Riva, Yoshiki Sekijima, Per Westermark, Shaji Kumar, Stefan Olaf Schönland
{"title":"Global patterns of amyloid typing: results of a survey by the International Society of Amyloidosis (ISA).","authors":"Hironobu Naiki, Mona Johnson, Kaylee Walters, Alexander Carpinteiro, M Teresa Cibeira, Anita D'Souza, Efstathios Kastritis, Mathew S Maurer, Lisa Mendelson, Giampaolo Merlini, Eli Muchtar, Giovanni Palladini, Eloisa Riva, Yoshiki Sekijima, Per Westermark, Shaji Kumar, Stefan Olaf Schönland","doi":"10.1080/13506129.2025.2462992","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Accurate tissue typing in amyloidosis is essential to provide appropriate therapy for individual patients.</p><p><strong>Objective: </strong>To get a real-life overview of typing strategies worldwide.</p><p><strong>Methods: </strong>The International Society of Amyloidosis (ISA) performed an online questionnaire survey among ISA members. We prepared questionnaire sheets for referral institutions (Category 1; C1), institutions performing amyloid typing for internal requests only (C2), and institutions outsourcing amyloid typing to referral institutions (C3), respectively.</p><p><strong>Results: </strong>Seventy-six institutions participated in this survey, including C1 (<i>n</i> = 33), C2 (<i>n</i> = 20) and C3 (<i>n</i> = 23) institutions. Multiple typing methods were available across the responding institutions, including immunohistochemistry (85% of C1/C2 institutions), immunofluorescence microscopy (43%), genetic analysis (77%) and mass spectrometric analysis (42%). Commercial antibodies were used worldwide for immunohistochemistry. C1 institutions in Europe and Asia also used various in-house antibodies. Ninety-three percent of institutions performed genetic analysis of <i>TTR gene</i>, followed by <i>APOA1</i> (43%), <i>FGA</i>, <i>GSN</i>, <i>LYZ</i> (33%) and <i>APOA2</i> (31%). Hierarchical referral flows of mass spectrometric analysis, immunohistochemistry and genetic analysis were observed regionally and internationally. Globalization and centralization of referral flows were more prominent for mass spectrometric analysis.</p><p><strong>Conclusion: </strong>These data provide an assessment of the current state, enabling improvement in capabilities of amyloid typing worldwide and enhancing regional/international networks.</p>","PeriodicalId":50964,"journal":{"name":"Amyloid-Journal of Protein Folding Disorders","volume":" ","pages":"1-6"},"PeriodicalIF":5.2000,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Amyloid-Journal of Protein Folding Disorders","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/13506129.2025.2462992","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Accurate tissue typing in amyloidosis is essential to provide appropriate therapy for individual patients.
Objective: To get a real-life overview of typing strategies worldwide.
Methods: The International Society of Amyloidosis (ISA) performed an online questionnaire survey among ISA members. We prepared questionnaire sheets for referral institutions (Category 1; C1), institutions performing amyloid typing for internal requests only (C2), and institutions outsourcing amyloid typing to referral institutions (C3), respectively.
Results: Seventy-six institutions participated in this survey, including C1 (n = 33), C2 (n = 20) and C3 (n = 23) institutions. Multiple typing methods were available across the responding institutions, including immunohistochemistry (85% of C1/C2 institutions), immunofluorescence microscopy (43%), genetic analysis (77%) and mass spectrometric analysis (42%). Commercial antibodies were used worldwide for immunohistochemistry. C1 institutions in Europe and Asia also used various in-house antibodies. Ninety-three percent of institutions performed genetic analysis of TTR gene, followed by APOA1 (43%), FGA, GSN, LYZ (33%) and APOA2 (31%). Hierarchical referral flows of mass spectrometric analysis, immunohistochemistry and genetic analysis were observed regionally and internationally. Globalization and centralization of referral flows were more prominent for mass spectrometric analysis.
Conclusion: These data provide an assessment of the current state, enabling improvement in capabilities of amyloid typing worldwide and enhancing regional/international networks.
期刊介绍：
Amyloid: the Journal of Protein Folding Disorders is dedicated to the study of all aspects of the protein groups and associated disorders that are classified as the amyloidoses as well as other disorders associated with abnormal protein folding. The journals major focus points are:
etiology,
pathogenesis,
histopathology,
chemical structure,
nature of fibrillogenesis;
whilst also publishing papers on the basic and chemical genetic aspects of many of these disorders.
Amyloid is recognised as one of the leading publications on amyloid protein classifications and the associated disorders, as well as clinical studies on all aspects of amyloid related neurodegenerative diseases and major clinical studies on inherited amyloidosis, especially those related to transthyretin. The Journal also publishes book reviews, meeting reports, editorials, thesis abstracts, review articles and symposia in the various areas listed above.
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