A comparison between adjuvant and delivering functions of iron oxide and calcium phosphate nanoparticles, using a model protein against Brucella melitensis.

IF 1.3 Q4 IMMUNOLOGY

Clinical and Experimental Vaccine Research Pub Date : 2025-01-01 Epub Date: 2025-01-13 DOI:10.7774/cevr.2025.14.e3

Tahereh Goudarzi, Morteza Abkar, Mahdi Fasihi-Ramandi, Mohammadsaleh Peikar, Zahra Zamanzadeh

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引用次数: 0

Abstract

Purpose: Calcium phosphate (CaP) and iron oxide (IO) nanoparticles (NPs) are promising adjuvants and delivery systems for vaccination. Furthermore, it has been shown that the chimeric antigen TF/Bp26/Omp31 (TBO) is a good candidate for stimulating protection against virulent Brucella melitensis. Our aim in the present study was to compare the roles of CaP and IO NPs for induction of the immune response and protection against B. melitensis 16M by using the TBO antigen as a model protein.

Materials and methods: The tbo gene was expressed in the bacterial host and was evaluated by SDS-PAGE and western blot. The recombinant TBO was loaded onto CaP (CaP/TBO) and IO (IO/TBO) NPs. CaP/TBO and IO/TBO NPs were administered subcutaneously.

Results: Antibody levels showed that immunization with both CaP/TBO and IO/TBO NPs stimulated mixed Th1-Th2 immune responses. In addition, immunized mice were challenged with a virulent strain of B. melitensis 16M. Immunized mice with CaP/TBO NPs showed a higher degree of protection than vaccinated animals with IO/TBO NPs.

Conclusion: Altogether, our results indicated that the CaP NPs are a potent adjuvant and delivery system for subcutaneously administered Brucella antigens.

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氧化铁和磷酸钙纳米颗粒的佐剂和递送功能的比较，使用抗布鲁氏菌模型蛋白。

目的：磷酸钙（CaP）和氧化铁（IO）纳米颗粒（NPs）是很有前途的疫苗佐剂和递送系统。此外，已经证明嵌合抗原TF/Bp26/Omp31 （TBO）是一个很好的候选物，可以刺激对毒力布鲁氏菌的保护。本研究的目的是以TBO抗原为模型蛋白，比较CaP和IO NPs在诱导猪B. melitensis 16M免疫应答和保护中的作用。材料与方法：在细菌宿主中表达tbo基因，采用SDS-PAGE和western blot检测。将重组TBO分别加载到CaP （CaP/TBO）和IO (IO/TBO) NPs上。皮下注射CaP/TBO和IO/TBO NPs。结果：抗体水平显示，CaP/TBO和IO/TBO两种NPs均可刺激混合Th1-Th2免疫反应。此外，免疫小鼠用毒力强的B. melitensis 16M毒株攻毒。CaP/TBO NPs免疫小鼠比IO/TBO NPs免疫小鼠具有更高的保护程度。结论：总的来说，我们的研究结果表明，CaP NPs是一种有效的皮下给药布鲁氏菌抗原的佐剂和递送系统。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical and Experimental Vaccine Research IMMUNOLOGY-

CiteScore

3.70

自引率

3.70%

发文量

审稿时长

8 weeks

期刊介绍： Clin Exp Vaccine Res, the official English journal of the Korean Vaccine Society, is an international, peer reviewed, and open-access journal. It covers all areas related to vaccines and vaccination. Clin Exp Vaccine Res publishes editorials, review articles, special articles, original articles, case reports, brief communications, and correspondences covering a wide range of clinical and experimental subjects including vaccines and vaccination for human and animals against infectious diseases caused by viruses, bacteria, parasites and tumor. The scope of the journal is to disseminate information that may contribute to elaborate vaccine development and vaccination strategies targeting infectious diseases and tumors in human and animals. Relevant topics range from experimental approaches to (pre)clinical trials for the vaccine research based on, but not limited to, basic laboratory, translational, and (pre)clinical investigations, epidemiology of infectious diseases and progression of all aspects in the health related issues. It is published printed and open accessed online issues (https://ecevr.org) two times per year in 31 January and 31 July. Clin Exp Vaccine Res is linked to many international databases and is made freely available to institutions and individuals worldwide