A TPR domain protein, OsTPR028, regulates grain size and weight in rice

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2025-02-07 DOI:10.1016/j.plantsci.2025.112405

Zongfei Zhang , Xin Wang , Yi Bao, Huihui Wang, Xin Yan, Pengfei Liao, Shaobo Li, Jiexiu Ouyang

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引用次数: 0

Abstract

Grain size and weight are critical determinants of rice yield and quality, yet their underlying genetic and regulatory mechanisms remain largely unexplored. In this study, we identified a protein containing TPR domain, named OsTPR028, which is localized in the cytoplasm. The gene encoding this protein is highly expressed during grain development. Homozygous ostpr028 mutant exhibited significant reductions in grain size, grain filling rate, and grain weight, accompanied by decreased levels of starch, amylose, and lipids. Transcriptomic and qRT-PCR analyses demonstrated that OsTPR028 regulates the expression of genes involved in starch biosynthesis and lipid transport. Protein interaction assays revealed that OsTPR028 interacts with an endosperm-specific protein, OsEnS45, whose knockout similarly resulted in reduced grain size and weight. Further investigations indicated that impaired spikelet cell division is the primary cause of these phenotypic defects in both ostpr028 and osens45 mutants. Together, our findings elucidate the critical role of the OsTPR028-OsEnS45 module in grain development and offer promising molecular targets for improving rice yield and quality through breeding programs.

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.