Albiflorin ameliorates neuroinflammation and exerts neuroprotective effects in Parkinson's disease models.

IF 2.9 4区 医学 Q3 IMMUNOLOGY
Yuan Gao, Yanmei Chen, Ning Wang, Qiang Meng
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引用次数: 0

Abstract

Background: Albiflorin isolated from Paeoniae Alba Radix can cross the blood-brain barrier (BBB) and possesses analgesia, anticonvulsant, anti-inflammatory, and hepatoprotective properties. This study investigates albiflorin functions and related mechanisms in Parkinson's disease (PD) pathogenesis.

Methods: Cellular and animal models of PD were constructed. Cell viability and apoptosis were detected by CCK-8 assays. Levels of Iba-1 and TH were measured by immunofluorescence staining, western blotting, and immunohistochemistry staining. Levels of pro-inflammatory mediators and pathway-related genes were measured by western blotting and RT-qPCR. Locomotor activity of mice was examined by open field test, rod climbing test, and rod rotating test.

Results: For in vitro analysis, albiflorin inhibited LPS-induced microglial activation and neuroinflammation. Additionally, albiflorin inactivated NF-κB and MAPK pathways in LPS-treated BV2 cells. Moreover, albiflorin attenuated neurotoxicity mediated by LPS-stimulated microglia. For in vivo analysis, albiflorin improved MPTP-induced locomotor activity deficits and reduced MPTP-induced dopaminergic neuron loss. In parallel, albiflorin inhibited activated microglia-mediated neuroinflammation in MPTP-treated mice.

Conclusion: Albiflorin mitigates neuronal apoptosis and improves behavioral impairments in MPTP-induced PD mouse model through inhibition of activated microglia-mediated neuroinflammation via the NF-κB and MAPK pathways.

Albiflorin在帕金森病模型中改善神经炎症并发挥神经保护作用。
背景:从芍药中分离得到的芍药苷能穿过血脑屏障(BBB),具有镇痛、抗惊厥、抗炎和保护肝脏的作用。本研究旨在探讨albiflorin在帕金森病(PD)发病中的作用及其相关机制。方法:建立PD细胞模型和动物模型。CCK-8检测细胞活力和凋亡。采用免疫荧光染色、免疫印迹和免疫组织化学染色检测血清Iba-1和TH水平。采用western blotting和RT-qPCR检测促炎介质和通路相关基因的水平。采用空地试验、爬杆试验和旋转杆试验检测小鼠的运动活动。结果:在体外分析中,albiflorin抑制lps诱导的小胶质细胞活化和神经炎症。此外,albiflorin在lps处理的BV2细胞中灭活NF-κB和MAPK通路。此外,albiflorin减轻了lps刺激的小胶质细胞介导的神经毒性。在体内分析中,albiflorin改善了mptp诱导的运动活动缺陷,减少了mptp诱导的多巴胺能神经元损失。同时,albiflorin抑制mptp处理小鼠激活的小胶质细胞介导的神经炎症。结论:Albiflorin通过NF-κB和MAPK通路抑制活化的小胶质细胞介导的神经炎症,减轻mptp诱导的PD小鼠模型神经元凋亡,改善行为障碍。
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来源期刊
CiteScore
5.40
自引率
0.00%
发文量
133
审稿时长
4-8 weeks
期刊介绍: The journal Immunopharmacology and Immunotoxicology is devoted to pre-clinical and clinical drug discovery and development targeting the immune system. Research related to the immunoregulatory effects of various compounds, including small-molecule drugs and biologics, on immunocompetent cells and immune responses, as well as the immunotoxicity exerted by xenobiotics and drugs. Only research that describe the mechanisms of specific compounds (not extracts) is of interest to the journal. The journal will prioritise preclinical and clinical studies on immunotherapy of disorders such as chronic inflammation, allergy, autoimmunity, cancer etc. The effects of small-drugs, vaccines and biologics against central immunological targets as well as cell-based therapy, including dendritic cell therapy, T cell adoptive transfer and stem cell therapy, are topics of particular interest. Publications pointing towards potential new drug targets within the immune system or novel technology for immunopharmacological drug development are also welcome. With an immunoscience focus on drug development, immunotherapy and toxicology, the journal will cover areas such as infection, allergy, inflammation, tumor immunology, degenerative disorders, immunodeficiencies, neurology, atherosclerosis and more. Immunopharmacology and Immunotoxicology will accept original manuscripts, brief communications, commentaries, mini-reviews, reviews, clinical trials and clinical cases, on the condition that the results reported are based on original, clinical, or basic research that has not been published elsewhere in any journal in any language (except in abstract form relating to paper communicated to scientific meetings and symposiums).
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