{"title":"A Turn-On Ratiometric Fluorescence Probe Based on Dye@MOFs for Visual Detection of AFB<sub>1</sub> with Paper Sensor Assisted by Smartphone.","authors":"Zhangmin Li, Zuopeng Li, Qianyi Li, Min Wang, Chang-Qing Miao, Yajia Liu, Sanbing Zhang","doi":"10.1007/s10895-025-04175-4","DOIUrl":null,"url":null,"abstract":"<p><p>Aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) is a potent carcinogen which threatens human health, therefore, rapid and efficient detection of AFB<sub>1</sub> is highly needed. Herein, a novel turn-on ratiometric fluorescent nanoprobe (RhB@NH<sub>2</sub>-MIL-53(Al)) was fabricated via a facial grinding for sensing AFB<sub>1</sub>. With increasing AFB<sub>1</sub> concentration, the fluorescence of Al-MOFs was enhanced due to molecular clamp action and electron transport, while the fluorescence of RhB remained almost unchanged, thus obtaining limit of detection (LOD) of 4.16 nM in the range of 0.005-2.56 µM. The sensor demonstrated excellent anti-interference capabilities and gave reasonable results when used to measure AFB<sub>1</sub> in grains. In addition, a paper-based portable sensor for AFB<sub>1</sub> detection was developed with a LOD of 29.89 nM in the range of 0.04-12.8 µM, while the colour changes from light pink to blue upon UV light exposure, making it a straightforward and effective technique for the on-site determination of AFB<sub>1</sub>.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Fluorescence","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1007/s10895-025-04175-4","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
Aflatoxin B1 (AFB1) is a potent carcinogen which threatens human health, therefore, rapid and efficient detection of AFB1 is highly needed. Herein, a novel turn-on ratiometric fluorescent nanoprobe (RhB@NH2-MIL-53(Al)) was fabricated via a facial grinding for sensing AFB1. With increasing AFB1 concentration, the fluorescence of Al-MOFs was enhanced due to molecular clamp action and electron transport, while the fluorescence of RhB remained almost unchanged, thus obtaining limit of detection (LOD) of 4.16 nM in the range of 0.005-2.56 µM. The sensor demonstrated excellent anti-interference capabilities and gave reasonable results when used to measure AFB1 in grains. In addition, a paper-based portable sensor for AFB1 detection was developed with a LOD of 29.89 nM in the range of 0.04-12.8 µM, while the colour changes from light pink to blue upon UV light exposure, making it a straightforward and effective technique for the on-site determination of AFB1.
期刊介绍：
Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
