Polyacrylamide-incorporated copper electrodes for electrochemical-colorimetric dual-mode detection of H₂O₂ released from tomato leaves.

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2025-02-08 DOI:10.1016/j.talanta.2025.127689

Linyan Su, Yuan Li, Huiyuan Chen, Xiao Liu, Zhiyao Zhang, Duan Bin, Beibei Yang, Lijun Sun, Hongbin Lu, Baohong Liu

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引用次数: 0

Abstract

Hydrogen peroxide (H₂O₂) plays a key role in a diverse array of cellular signaling pathways, which is closely related to plant health and physiological status. The accurate and efficient monitoring of H₂O₂ in living plant cells has attracted enormous interest. Herein, we developed an electrochemical-colorimetric dual-mode sensor based on the peroxidase-like activity of a polyacrylamide (PAM) -modified copper electrode (Cu-PAM), allowing for the in situ detection of H₂O₂ released from tomato leaves. The co-electrodeposition of unique combination of Cu and polyacrylamide, as well as the active site structure of Cu-centered peroxidases, can enhance adsorption performance by the hydrogen bonds between PAM with H₂O₂, which exhibited excellent electrochemical performance with a low limit of detection (LOD) of 0.0167 mM and a detection range of 0.05-25.31 mM. Meanwhile, a colorimetric signal output of the sensor that can be quantified from 1 μM to 70 μM with a LOD value of 0.33 μM. This work demonstrates a huge potential application prospect of the polyacrylamide-modified copper in the field of biosensors.

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.