MXene-mediated fluorescent recombinase polymerase amplification for rapid and sensitive DNA methylation biosensing.

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2025-05-15 Epub Date: 2025-02-03 DOI:10.1016/j.talanta.2025.127685

Bingshan Guo, Jiaoying Peng, Yongzhi Wang, Ying Chen, Ran Li, Xuemei Xu, Yongchao Yao, Wenchuang Walter Hu

引用次数: 0

Abstract

DNA methylation serves as a promising biomarker for early cancer detection, with its assessment providing crucial insights for prognosis and diagnosis. Herein, we present a novel MXene-mediated fluorescent recombinase polymerase amplification (RPA) strategy that achieves rapid and sensitive non-small cell lung cancer (NSCLC)-associated methylated DNA detection. Remarkably, MXene can effectively quench the fluorescence of fluorescein amidate (FAM) labeled primer, with fluorescence recovery occurring as primers extend to double-stranded DNA and are released during the RPA process. This method exhibits exceptional sensitivity and selectivity, enabling the detection of methylated DNA at concentrations as low as 10 fM, with assay completion achieved in less than 1 h. Its ability to precisely evaluate gene-specific methylation showcases its potential to advance methylation as a disease biomarker, particularly in NSCLC.

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.