{"title":"FeCuS multilayer nanoflowers loaded with N-CDs dual-mode method for the detection of uric acid and gout risk prediction with the aid of chemometrics.","authors":"Rou Cheng, Chunyan Yan, Zhengyue Xiao, Xiaomin Tang, Peng Xu, Ping Qiu","doi":"10.1016/j.talanta.2025.127700","DOIUrl":null,"url":null,"abstract":"<p><p>Transition metal sulfides have attracted much attention due to their low cost, high stability, adjustable performance, diverse morphology and good catalytic activity. In this work, nitrogen-doped carbon dots (N-CDs) were loaded onto bimetallic sulfides (FeCuS) and FeCuS/N-CDs could act as catalyst and fluorescent probe, which applied to the detection of uric acid (UA) in human blood and urine using dual-model assay (colorimetry and ratiometric fluorescence). The nanozyme has excellent peroxidase-like activity and steady-state kinetic results showed that the material prepared under optimized conditions had better affinity (K<sub>m</sub> = 0.42 mM) for the substrate and faster reaction rate (V<sub>max</sub> = 14.1 × 10<sup>-8</sup> M s<sup>-1</sup>). •OH and O<sub>2</sub><sup>•-</sup> were involved in the catalytic process of o-phenylenediamine (OPD) by FeCuS/N-CDs nanozymes. A profound discussion and quantitative analysis about the sensing mechanism of UA and fluorescence quenching mechanism between FeCuS/N-CDs and diaminophenazine (DAP) was performed. According to Parker equation, the inhibition efficiency of DAP in FeCuS/N-CDs was as high as 83 % of the total inhibition efficiency, confirming that the inhibition efficiency mainly came from internal filtering effect (IFE). The detection limits of ratiometric fluorescence method and colorimetric method were as low as 0.13 μM and 0.27 μM, respectively. Finally, principal component analysis was used to successfully distinguish three groups of people (healthy people, potential people and gout patients) and the model has potential value in clinical applications.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"287 ","pages":"127700"},"PeriodicalIF":5.6000,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Talanta","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.talanta.2025.127700","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Transition metal sulfides have attracted much attention due to their low cost, high stability, adjustable performance, diverse morphology and good catalytic activity. In this work, nitrogen-doped carbon dots (N-CDs) were loaded onto bimetallic sulfides (FeCuS) and FeCuS/N-CDs could act as catalyst and fluorescent probe, which applied to the detection of uric acid (UA) in human blood and urine using dual-model assay (colorimetry and ratiometric fluorescence). The nanozyme has excellent peroxidase-like activity and steady-state kinetic results showed that the material prepared under optimized conditions had better affinity (Km = 0.42 mM) for the substrate and faster reaction rate (Vmax = 14.1 × 10-8 M s-1). •OH and O2•- were involved in the catalytic process of o-phenylenediamine (OPD) by FeCuS/N-CDs nanozymes. A profound discussion and quantitative analysis about the sensing mechanism of UA and fluorescence quenching mechanism between FeCuS/N-CDs and diaminophenazine (DAP) was performed. According to Parker equation, the inhibition efficiency of DAP in FeCuS/N-CDs was as high as 83 % of the total inhibition efficiency, confirming that the inhibition efficiency mainly came from internal filtering effect (IFE). The detection limits of ratiometric fluorescence method and colorimetric method were as low as 0.13 μM and 0.27 μM, respectively. Finally, principal component analysis was used to successfully distinguish three groups of people (healthy people, potential people and gout patients) and the model has potential value in clinical applications.
期刊介绍：
Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome.
Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.
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