Bioactive Peptides From Rice Wine Subjected to a Simulation Digestion: Inhibition of α-Glucosidase and Enhancement of Insulin Sensitivity

Abstract

The study investigated the blood glucose regulation potentials of rice wine peptides. α-Glucosidase inhibitory activity of simulated digested rice wine was identified by in vivo experiments. After rigorous identification, screening, and validation, two principal functional peptides, LLGDV and NLDSS, were identified as the main contributors to the observed bioactivity of digested rice wine. Molecular docking and dynamics experiments found these peptides were demonstrated to occupy the active pocket of α-glucosidase via hydrogen bonds, van der Waals’ forces, and pi-bonding, effectively inhibiting the enzyme and reducing glucose absorption. Additional cellular experiments were performed to assess the peptides’ effects on cellular glucose metabolism. The outcomes indicated that peptide LLGDV significantly promoted glucose digestion in cells, pointing to its potential in enhancing insulin sensitivity and alleviating insulin resistance. The study’s results enhanced the scientific comprehension of rice wine health-promoting attributes and facilitated the creation of innovative health products. The identification and characterization of the bioactive peptides LLGDV and NLDSS establish a groundwork for forthcoming research, targeting the therapeutic potential of these peptides for diabetes and associated metabolic conditions management.