Regulation pattern of carotenoid biosynthesis induced by low light stress in Dunaliella

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology Pub Date : 2025-02-08 DOI:10.1016/j.biortech.2025.132206

Hao-Hong Chen , Jing-Xuan Wu , Rui-qi Chen , Sacha Fouquay O’Donnell , Ming-Hua Liang , Jian-Guo Jiang

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Abstract

This research aims to explore the response mechanism of carotenoid synthesis in Dunaliella bardawil under low light stress (≤13.5 µmol·m⁻²·s⁻¹). We analyzed carotenoid composition and its correlation with carotenogenic genes (CRTs) differential expression, promoter regulatory elements and transcription factors. DbPDS and DbZDS were significantly up-regulated under low light stress. Mutation experiments showed ACE, ASF1, CCACA-box, and CCAAT-box in DbZDS promoter key for low light regulation. In addition, CONSTANS (DbCO) and nuclear transcription factor YB (DbNF-YB) had a weak interaction and could bind to DbZDS gene promoter, whereas there was no interaction between DbZDS promoter and DbHY5 (ELONGATED HYPOCOTYL 5). We constructed a DbCO gene overexpressing strain, which significantly enhanced carotenoid content, notably increasing β-carotene levels to 2.3 times. These results significantly enhance our understanding of the regulation of low light stress in microalgae.

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来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.