Molecular characterization of putative antibiotic resistance determinant and virulence factors genes of Acinetobacter baumannii strains isolated from intensive care unit patients in Riyadh, Saudi Arabia

IF 4.7 3区医学 Q1 INFECTIOUS DISEASES

Journal of Infection and Public Health Pub Date : 2025-02-07 DOI:10.1016/j.jiph.2025.102695

Faris A. Alanazi , Khalid S. Almaary , Turki M. Dawoud , Mohamed A. El-Tayeb , Yahya B. Elbadawi , Ayman S. Mubarak , Ali M. Somily

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引用次数: 0

Abstract

Background

Multidrug resistance in Acinetobacter baumannii (A. baumannii) is a major global health threat. The resistance in A. baumannii is attributed to numerous factors, including antimicrobial resistance and virulence-determinant genes. Hence, the present study aimed to screen antibiotic resistance and virulence factors genes in 67 A. baumannii strains isolated from patients admitted to several hospitals in Riyadh City, Saudi Arabia.

Method

The A. baumannii isolates were confirmed by the VITEK-2 automated system and 16S rRNA phylogenetic relatedness. The phenotypic and genotypic resistance patterns of these isolates were also analyzed using conventional and molecular methods.

Results

Our finding showed that 94 % of isolated strains were MDR, and more than 94 % were resistant to the β-lactams group, particularly carbapenems, and the ciprofloxacin group. An intermediate resistance pattern was obtained with trimethoprim-sulfamethoxazole (71.6 %) and gentamicin (59.7 %). The lowest resistant patterns showed with colistin (5 %). The distribution of of resistance genes oxa-23, imp-like, oxa-24-like, tem, oxa-40, carb, oxa-58, floR, dfrA1 and qnrS were 100 %, 76.1 %, 64.1 %, 25.3 %, 23.8 %, 16.4 %, 14.9 %, 44.7 %, 5.9 % and 2.9 %, respectively. The distribution of virulence genes baP, pld, paaE, and surA1 was 98.5 %, followed by basD, traT, Omp33–36, and bauA were 97 %, 77.6 %, 74.4 %, and 64.1 %, respectively.

Conclusion

The study showed a strong relationship between virulence factors, especially biofilm formation, and antibiotic resistance.

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来源期刊

Journal of Infection and Public Health PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH -INFECTIOUS DISEASES

CiteScore

13.10

自引率

1.50%

发文量

203

审稿时长

96 days

期刊介绍： The Journal of Infection and Public Health, first official journal of the Saudi Arabian Ministry of National Guard Health Affairs, King Saud Bin Abdulaziz University for Health Sciences and the Saudi Association for Public Health, aims to be the foremost scientific, peer-reviewed journal encompassing infection prevention and control, microbiology, infectious diseases, public health and the application of healthcare epidemiology to the evaluation of health outcomes. The point of view of the journal is that infection and public health are closely intertwined and that advances in one area will have positive consequences on the other. The journal will be useful to all health professionals who are partners in the management of patients with communicable diseases, keeping them up to date. The journal is proud to have an international and diverse editorial board that will assist and facilitate the publication of articles that reflect a global view on infection control and public health, as well as emphasizing our focus on supporting the needs of public health practitioners. It is our aim to improve healthcare by reducing risk of infection and related adverse outcomes by critical review, selection, and dissemination of new and relevant information in the field of infection control, public health and infectious diseases in all healthcare settings and the community.