DNA data (genome skims and metabarcodes) paired with chemical data demonstrate utility for retrospective analysis of forage linked to fatal poisoning of cattle

IF 2.6 4区医学 Q2 PHARMACOLOGY & PHARMACY

Toxicon Pub Date : 2025-02-05 DOI:10.1016/j.toxicon.2025.108285

Daniel Cook , Brandon Kocurek , Clint Stonecipher , Kevin D. Welch , Dale R. Gardner , Mark Mammel , Elizabeth Reed , Padmini Ramachandran , David Erickson , Seth Commichaux , Andrea Ottesen

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引用次数: 0

Abstract

Prepared and stored feeds, fodder, silage, and hay may be contaminated by toxic plants resulting in the loss of livestock. Several poisonous plants have played significant roles in livestock deaths from forage consumption in recent years in the Western United States including Salvia reflexa. Metagenomic data, genome skims and metabarcodes, have been used for identification and characterization of plants in complex botanical matrices including diet composition of animals, mixed forages, and herbal products. Here, chemistry, genome skims, and metabarcoding were used to retrospectively describe the composition of contaminated alfalfa hay from a case of Salvia reflexa (lanceleaf sage) poisoning that killed 165 cattle. Genome skims and metabarcoding provided similar estimates of the relative abundance of the Salvia in the hay samples when compared to chemical methods. Additionally, genome skims and metabarcoding provided similar estimates of species composition in the contaminated hay and rumen contents of poisoned animals. The data demonstrate that genome skims and DNA metabarcoding may provide useful tools for plant poisoning investigations.

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DNA数据（基因组图谱和元条形码）与化学数据相结合，证明了对与牛致命中毒有关的饲料进行回顾性分析的实用性。

制备和储存的饲料、饲料、青贮饲料和干草可能被有毒植物污染，导致牲畜死亡。近年来，包括鼠尾草在内的几种有毒植物在美国西部牧草消费导致的牲畜死亡中发挥了重要作用。元基因组数据、基因组图谱和元条形码已被用于鉴定和表征复杂植物基质中的植物，包括动物的日粮组成、混合饲料和草药产品。在这里，化学、基因组图谱和元条形码被用来回顾性地描述从导致165头牛死亡的鼠尾草中毒事件中污染的苜蓿干草的成分。与化学方法相比，基因组切片和元条形码提供了干草样品中鼠尾草相对丰度的相似估计。此外，基因组图谱和元条形码提供了污染干草和中毒动物瘤胃内容物中物种组成的类似估计。这些数据表明，基因组图谱和DNA元条形码可能为植物中毒调查提供有用的工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Toxicon 医学-毒理学

CiteScore

4.80

自引率

10.70%

发文量

358

审稿时长

68 days

期刊介绍： Toxicon has an open access mirror Toxicon: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. An introductory offer Toxicon: X - full waiver of the Open Access fee. Toxicon''s "aims and scope" are to publish: -articles containing the results of original research on problems related to toxins derived from animals, plants and microorganisms -papers on novel findings related to the chemical, pharmacological, toxicological, and immunological properties of natural toxins -molecular biological studies of toxins and other genes from poisonous and venomous organisms that advance understanding of the role or function of toxins -clinical observations on poisoning and envenoming where a new therapeutic principle has been proposed or a decidedly superior clinical result has been obtained. -material on the use of toxins as tools in studying biological processes and material on subjects related to venom and antivenom problems. -articles on the translational application of toxins, for example as drugs and insecticides -epidemiological studies on envenoming or poisoning, so long as they highlight a previously unrecognised medical problem or provide insight into the prevention or medical treatment of envenoming or poisoning. Retrospective surveys of hospital records, especially those lacking species identification, will not be considered for publication. Properly designed prospective community-based surveys are strongly encouraged. -articles describing well-known activities of venoms, such as antibacterial, anticancer, and analgesic activities of arachnid venoms, without any attempt to define the mechanism of action or purify the active component, will not be considered for publication in Toxicon. -review articles on problems related to toxinology. To encourage the exchange of ideas, sections of the journal may be devoted to Short Communications, Letters to the Editor and activities of the affiliated societies.