Mengjun Ge, Yunrui Xing, Yaning Sun, Wenjin Cai, Yao Wang, Xiaofei Hu
{"title":"Establishment of a Chemiluminescent ELISA Method for Florfenicol in Eggs and Chicken Meat","authors":"Mengjun Ge, Yunrui Xing, Yaning Sun, Wenjin Cai, Yao Wang, Xiaofei Hu","doi":"10.1007/s12161-024-02690-x","DOIUrl":null,"url":null,"abstract":"<div><p>To establish a highly sensitive chemiluminescent enzyme-linked immunosorbent assay (ci-ELISA) method for the determination of florfenicol (FF) in egg and chicken meat. Based on the obtained monoclonal antibodies against florfenicol, a chessboard test was employed to determine the optimal working concentration of artificial antigen and antibody, and then established a ci-ELISA method, and characterized its performance. The results showed that the ci-ELISA method had a detection limit of 5.433 pg/mL, an IC<sub>50</sub> of 30.893 pg/mL, and a detection range between 5.433 and 193.577 pg/mL. The coefficient of variation was below 10%, indicating satisfactory precision. The spiked recoveries in egg samples were between 90.03% and 95.24%, and the spiked recoveries in chicken meat samples were between 90.56% and 93.04%. In conclusion, the developed ci-ELISA method is highly sensitive and suitable for monitoring and detection of trace amounts of FF residues in eggs and chicken meat.</p></div>","PeriodicalId":561,"journal":{"name":"Food Analytical Methods","volume":"18 1","pages":"48 - 56"},"PeriodicalIF":2.6000,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Analytical Methods","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1007/s12161-024-02690-x","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
To establish a highly sensitive chemiluminescent enzyme-linked immunosorbent assay (ci-ELISA) method for the determination of florfenicol (FF) in egg and chicken meat. Based on the obtained monoclonal antibodies against florfenicol, a chessboard test was employed to determine the optimal working concentration of artificial antigen and antibody, and then established a ci-ELISA method, and characterized its performance. The results showed that the ci-ELISA method had a detection limit of 5.433 pg/mL, an IC50 of 30.893 pg/mL, and a detection range between 5.433 and 193.577 pg/mL. The coefficient of variation was below 10%, indicating satisfactory precision. The spiked recoveries in egg samples were between 90.03% and 95.24%, and the spiked recoveries in chicken meat samples were between 90.56% and 93.04%. In conclusion, the developed ci-ELISA method is highly sensitive and suitable for monitoring and detection of trace amounts of FF residues in eggs and chicken meat.
期刊介绍：
Food Analytical Methods publishes original articles, review articles, and notes on novel and/or state-of-the-art analytical methods or issues to be solved, as well as significant improvements or interesting applications to existing methods. These include analytical technology and methodology for food microbial contaminants, food chemistry and toxicology, food quality, food authenticity and food traceability. The journal covers fundamental and specific aspects of the development, optimization, and practical implementation in routine laboratories, and validation of food analytical methods for the monitoring of food safety and quality.
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