Exploring the allometry between ear saturated water accumulation and dry mass for diagnosing winter wheat water status during the reproductive growth

Abstract

The ear, which begins to form and develop during the reproductive growth phase, relies on maintaining a normal water status for its formation, grain filling, and overall yield. Accurate diagnosis of water status during the reproductive growth phase is imperative for achieving precision water management in winter wheat cultivation. Previous studies used the allometric relationship between plant dry mass (PDM) and plant saturated water accumulation (SWA_P) to develop critical SWA_P curves, which were employed to assess the water status of winter wheat and maize during their vegetative growth phase. However, it remains uncertain whether this method is applicable to the ear of winter wheat during its reproductive growth phase. The study focused on developing and validating a model to quantify the water status of winter wheat during reproductive growth phase by using critical ear saturated water accumulation (SWA_E) curves and water diagnostic index (WDI) based on ear, and to analyze the effect of water-nitrogen interaction on it. Field experiments involving four water and two nitrogen treatments were conducted from 2019 to 2023 to determine the relationship between ear dry mass (EDM) and SWA_E during the reproductive growth phase of winter wheat. The impact of water-nitrogen interaction on EDM-SWA_E allometry was also analyzed. In addition, the ear WDI was defined as the ratio of the actual SWA_E value to the critical SWA_E value under the same EDM. The critical SWA_E curves under nitrogen limited (N1) and non-nitrogen limited (N2) conditions were constructed (N1: SWA_E = 3.53EDM^0.48; N2: SWA_E = 4.53EDM^0.47). Nitrogen deficiency lowered the SWA_E value at the same EDM, but it did not impact its accumulation rate. The indirect soil nitrogen deficiency, reduction of grain number per ear and early grain filling caused by drought were the three main factors leading to the decrease of ear WDI. The ear WDI effectively distinguishes varying degrees of water stress; however, it is essential to minimize errors resulting from its uncertainty before application. These findings will provide valuable insights into the water status of winter wheat under varying water and nitrogen conditions during the reproductive growth phase. Additionally, they will serve as a foundation for advancing future research on precise irrigation strategies.