Aldehyde Dehydrogenase from Klebsiella pneumoniae: A Robust Biocatalyst for Preparing Heteroatom-Containing Carboxylic Acids

IF 4.4 2区化学 Q2 CHEMISTRY, APPLIED

Advanced Synthesis & Catalysis Pub Date : 2025-02-07 DOI:10.1002/adsc.202500027

Yuchen Han, Nan Geng, Jiangtao Sha, Huanhuan Li, Chun You, Weidong Liu, Jie Zhang, Jianjun Shi, Xin Wu, Wuyuan Zhang

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引用次数: 0

Abstract

Heterocyclic scaffolds have broad applications in organic synthesis, resulting in the production of essential compounds utilized in pharmaceuticals, agrochemicals, and dietary products. In this study, we present the characterization of a discovered succinic semialdehyde dehydrogenase from Klebsiella pneumoniae (KpSSADH) and elucidate its crystallographic structure. Further investigation into the catalytic performance of KpSSADH reveals its remarkable efficiency in converting various heteroatomcontaining (including N, S, O, and their combinations) cyclic aldehydes into the corresponding acids with conversions reaching up to 99%. To expand the range of available substrates, we designed cascade reactions by integrating thiol oxidase from Methylovorus sp. MP688 or unspecific peroxygenase from Agrocybe aegerita with KpSSADH. Through this approach, the desired acids via alcohol oxidation and C-H bond oxyfunctionalization were obtained, respectively. The discovery of KpSSADH and the envisioned cascade reactions have significantly broadened the biocatalytic toolbox for synthesizing heteroatom-containing carboxylic acids, thereby holding potential for applications in the modern pharmaceutical industry.

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来源期刊

Advanced Synthesis & Catalysis 化学-应用化学

CiteScore

9.40

自引率

7.40%

发文量

447

审稿时长

1.8 months

期刊介绍： Advanced Synthesis & Catalysis (ASC) is the leading primary journal in organic, organometallic, and applied chemistry. The high impact of ASC can be attributed to the unique focus of the journal, which publishes exciting new results from academic and industrial labs on efficient, practical, and environmentally friendly organic synthesis. While homogeneous, heterogeneous, organic, and enzyme catalysis are key technologies to achieve green synthesis, significant contributions to the same goal by synthesis design, reaction techniques, flow chemistry, and continuous processing, multiphase catalysis, green solvents, catalyst immobilization, and recycling, separation science, and process development are also featured in ASC. The Aims and Scope can be found in the Notice to Authors or on the first page of the table of contents in every issue.