[Preliminary study on the role of siglec-9 expression in peripheral blood of acute respiratory distress syndrome patients].

Abstract

Objective: Sialic acid-binding immunoglobulin-like lectin (siglec)-9 is a type Ⅰ transmembrane protein that plays an important role in intrinsic immunity. In this study, we examined the siglec-9 expression levels in mononuclear macrophages, neutrophils and plasma from peripheral blood of acute respiratory distress syndrome (ARDS) patients and investigated its clinical value. Methods: Peripheral blood was obtained from ARDS patients (n=54) admitted to the Department of Respiratory and Critical Care Medicine, Nanjing Jinling Hospital and the Second People's Hospital of Wuhu, Anhui Province, China, from July 2021 to October 2022. Healthy volunteers (n=50) from the physical examination center and hospitalized patients (n=47) with lung infection were included as control groups. The expression of cellular and plasma siglec-9 was detected by flow cytometry and ELISA separately. Results: Compared with lung infection group and healthy control group, the expression of monocytes and plasma siglec-9 in ARDS group increased significantly. But there was no significant difference in the expression of monocytes and plasma siglec-9 between lung infection group and healthy control group. The level of plasma siglec-9 was significantly higher in moderate-severe ARDS patients than that in mild patients (P<0.05). Multivariable logistic regression analysis showed that IL-6, LDH and plasma siglec-9 were independent risk factors of ARDS, while plasma siglec-9, IL-6 and APACHE Ⅱ were independent risk factors of moderate-severe ARDS. Conclusion: The expression of siglec-9 in the plasma of ARDS patients is positively correlated with the severity of ARDS, which has a certain value in the assessment of ARDS.