Yueju Pill Inhibits Apoptosis by Regulating the SCF/c-Kit/PI3K/AKT Signaling Pathway to Ameliorate Functional Dyspepsia.

IF 1.6 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Combinatorial chemistry & high throughput screening Pub Date : 2025-02-04 DOI:10.2174/0113862073344555241120103257

Yaru Gu, Yaning Biao, Chenxu Liu, Yufang Zhang, Ya Gao, Yucong Xue, Yixin Zhang

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引用次数: 0

Abstract

Objective: This study aimed to investigate the therapeutic effect of Yueju Pill (YJP) on functional dyspepsia (FD) rats and its mechanism of promoting gastrointestinal motility.

Methods: We replicated FD rat models using classical tail pinching and irregular feeding for 14 days. After 28 days of YJP treatment, we measured the gastric emptying rate and intestinal propulsion rate of the rats. Hematoxylin-eosin (H&E) staining was used to observe the pathological damage in the gastric antrum. The serum levels of related brain-gut peptides (BGPs) were determined using the enzyme-linked immunosorbent assay (ELISA). Furthermore, we detected the expression of proteins related to the SCF/c-Kit/PI3K/AKT signaling pathway through Western blot and immunohistochemistry. Finally, we assessed the levels of apoptosis using the TUNEL assay.

Results: YJP improved gastric emptying and small intestine propulsion rates while reducing gastric tissue injury in FD rats. Moreover, YJP increased the levels of gastrin (GAS) and ghrelin (Ghrelin) and decreased the levels of cholecystokinin (CCK) and vasoactive intestinal peptide (VIP). YJP also elevated the levels of SCF, c-kit and Bcl-2, promoted the phosphorylation of PI3K and AKT, and inhibited the expression of Bax.

Conclusion: YJP achieved the effect of FD treatment by regulating the SCF/c-Kit/PI3K/AKT pathway, providing a theoretical basis for the clinical treatment of FD.

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来源期刊

Combinatorial chemistry & high throughput screening 化学-生化研究方法

CiteScore

3.10

自引率

5.60%

发文量

327

审稿时长

7.5 months

期刊介绍： Combinatorial Chemistry & High Throughput Screening (CCHTS) publishes full length original research articles and reviews/mini-reviews dealing with various topics related to chemical biology (High Throughput Screening, Combinatorial Chemistry, Chemoinformatics, Laboratory Automation and Compound management) in advancing drug discovery research. Original research articles and reviews in the following areas are of special interest to the readers of this journal: Target identification and validation Assay design, development, miniaturization and comparison High throughput/high content/in silico screening and associated technologies Label-free detection technologies and applications Stem cell technologies Biomarkers ADMET/PK/PD methodologies and screening Probe discovery and development, hit to lead optimization Combinatorial chemistry (e.g. small molecules, peptide, nucleic acid or phage display libraries) Chemical library design and chemical diversity Chemo/bio-informatics, data mining Compound management Pharmacognosy Natural Products Research (Chemistry, Biology and Pharmacology of Natural Products) Natural Product Analytical Studies Bipharmaceutical studies of Natural products Drug repurposing Data management and statistical analysis Laboratory automation, robotics, microfluidics, signal detection technologies Current & Future Institutional Research Profile Technology transfer, legal and licensing issues Patents.