Study on the Mechanism of Anti-Atopic Dermatitis by Herba Siegesbeckiae Based on Metabolomics.

IF 1.6 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Combinatorial chemistry & high throughput screening Pub Date : 2025-02-04 DOI:10.2174/0113862073345094241226112758

YingYue Wang, Xiaowei Chen, Lingling Zhang, Yuting Chen, Yubin Xu, Chunxue You, Xuetao Lu

{"title":"Study on the Mechanism of Anti-Atopic Dermatitis by Herba Siegesbeckiae Based on Metabolomics.","authors":"YingYue Wang, Xiaowei Chen, Lingling Zhang, Yuting Chen, Yubin Xu, Chunxue You, Xuetao Lu","doi":"10.2174/0113862073345094241226112758","DOIUrl":null,"url":null,"abstract":"Background: Atopic dermatitis is a common inflammatory skin disease worldwide that is characterized by skin barrier dysfunction, itching, and a reduced quality of life.Objective: The research at hand aimed to delve into the anti-atopic dermatitis mechanism of Herba Siegesbeckiae, a traditional medicinal herb, using a metabolomic approach.Methods: The molecular mechanism by which Herba Siegesbeckiae acts against atopic dermatitis was investigated by establishing a mouse model of atopic dermatitis while conducting a metabolomics analysis on its metabolites.Results: Interleukin IL-13, IL-17A, IL-3, IL-31, IL-33, IL4, IL-5, TSLP, IgE, and histamine levels in serum, participating in inhibiting itching and regulating immunity signaling were found to be restored to varying degrees in AD treating with HS. A total of 31 differential metabolites were selected from metabolomics results, among which N-acetyl-L-alanine (VIP = 1.62), Nacetyl- L-methionine (VIP = 1.5), uracil (VIP = 1.47), and prostaglandin E2 (VIP = 1.4) play important roles in the anti-AD regulatory mechanisms of HS and can be used as biomarkers. In addition, the mechanisms of HS anti-AD have been shown to be associated with seven metabolic pathways, including β-alanine metabolism, glycerophospholipid metabolism, histidine metabolism, and so on.Conclusion: In conclusion, HS demonstrated properties that counteract Atopic Dermatitis by suppressing itchiness and boosting the immune system, subsequently controlling the concentrations of related metabolites.","PeriodicalId":10491,"journal":{"name":"Combinatorial chemistry & high throughput screening","volume":" ","pages":""},"PeriodicalIF":1.6000,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Combinatorial chemistry & high throughput screening","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2174/0113862073345094241226112758","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Atopic dermatitis is a common inflammatory skin disease worldwide that is characterized by skin barrier dysfunction, itching, and a reduced quality of life.

Objective: The research at hand aimed to delve into the anti-atopic dermatitis mechanism of Herba Siegesbeckiae, a traditional medicinal herb, using a metabolomic approach.

Methods: The molecular mechanism by which Herba Siegesbeckiae acts against atopic dermatitis was investigated by establishing a mouse model of atopic dermatitis while conducting a metabolomics analysis on its metabolites.

Results: Interleukin IL-13, IL-17A, IL-3, IL-31, IL-33, IL4, IL-5, TSLP, IgE, and histamine levels in serum, participating in inhibiting itching and regulating immunity signaling were found to be restored to varying degrees in AD treating with HS. A total of 31 differential metabolites were selected from metabolomics results, among which N-acetyl-L-alanine (VIP = 1.62), Nacetyl- L-methionine (VIP = 1.5), uracil (VIP = 1.47), and prostaglandin E2 (VIP = 1.4) play important roles in the anti-AD regulatory mechanisms of HS and can be used as biomarkers. In addition, the mechanisms of HS anti-AD have been shown to be associated with seven metabolic pathways, including β-alanine metabolism, glycerophospholipid metabolism, histidine metabolism, and so on.

Conclusion: In conclusion, HS demonstrated properties that counteract Atopic Dermatitis by suppressing itchiness and boosting the immune system, subsequently controlling the concentrations of related metabolites.

查看原文本刊更多论文

基于代谢组学的白桦抗特应性皮炎机制研究。

背景：特应性皮炎是一种世界范围内常见的炎症性皮肤病，其特征是皮肤屏障功能障碍、瘙痒和生活质量下降。目的：利用代谢组学方法，探讨传统中草药白荆抗特应性皮炎的作用机制。方法：通过建立特应性皮炎小鼠模型，并对其代谢产物进行代谢组学分析，探讨白荆抗特应性皮炎的分子机制。结果：HS治疗AD后，参与抑制瘙痒和调节免疫信号的血清白细胞介素IL-13、IL-17A、IL-3、IL-31、IL-33、il - 4、IL-5、TSLP、IgE、组胺水平均有不同程度恢复。从代谢组学结果中共筛选出31个差异代谢物，其中n -乙酰基- l-丙氨酸（VIP = 1.62）、Nacetyl- l-蛋氨酸（VIP = 1.5）、尿嘧啶（VIP = 1.47）和前列腺素E2 （VIP = 1.4）在HS抗ad调控机制中发挥重要作用，可作为生物标志物。此外，HS抗ad的机制已被证明与β-丙氨酸代谢、甘油磷脂代谢、组氨酸代谢等7种代谢途径有关。结论：综上所述，HS通过抑制瘙痒和增强免疫系统，从而控制相关代谢物的浓度来对抗特应性皮炎。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Combinatorial chemistry & high throughput screening 化学-生化研究方法

CiteScore

3.10

自引率

5.60%

发文量

327

审稿时长

7.5 months

期刊介绍： Combinatorial Chemistry & High Throughput Screening (CCHTS) publishes full length original research articles and reviews/mini-reviews dealing with various topics related to chemical biology (High Throughput Screening, Combinatorial Chemistry, Chemoinformatics, Laboratory Automation and Compound management) in advancing drug discovery research. Original research articles and reviews in the following areas are of special interest to the readers of this journal: Target identification and validation Assay design, development, miniaturization and comparison High throughput/high content/in silico screening and associated technologies Label-free detection technologies and applications Stem cell technologies Biomarkers ADMET/PK/PD methodologies and screening Probe discovery and development, hit to lead optimization Combinatorial chemistry (e.g. small molecules, peptide, nucleic acid or phage display libraries) Chemical library design and chemical diversity Chemo/bio-informatics, data mining Compound management Pharmacognosy Natural Products Research (Chemistry, Biology and Pharmacology of Natural Products) Natural Product Analytical Studies Bipharmaceutical studies of Natural products Drug repurposing Data management and statistical analysis Laboratory automation, robotics, microfluidics, signal detection technologies Current & Future Institutional Research Profile Technology transfer, legal and licensing issues Patents.