Taylah J Johnston, Allison J Stewart, Elizabeth L Dryburgh, François-René Bertin
{"title":"Time but not storage of equine plasma in silicate-coated tubes affects adrenocorticotropic hormone stability.","authors":"Taylah J Johnston, Allison J Stewart, Elizabeth L Dryburgh, François-René Bertin","doi":"10.2460/ajvr.24.11.0362","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Pituitary pars intermedia dysfunction (PPID) is diagnosed by measuring the ACTH concentrations. Due to the reported instability of ACTH, it is recommended to transfer centrifuged plasma into cryovials; however, in practice, cryovials are infrequently used, and serum (red-top) tubes are used instead. This study investigated whether this procedure affects ACTH concentrations and the diagnosis of PPID.</p><p><strong>Methods: </strong>This was a cohort study. Blood was collected into EDTA tubes from 9 horses with PPID and 7 controls. After centrifugation, plasma was either aliquoted into a cryovial or into a serum tube. Samples were stored at 4 and 20 °C with ACTH concentrations measured using a chemiluminescent assay at 2, 24, and 48 hours after collection. Data were analyzed using a 2-way repeated measures ANOVA with P < .05 considered significant.</p><p><strong>Results: </strong>There was a significant effect of time on ACTH concentrations in horses with PPID at 4 and 20 °C and in all horses at 20 °C with decreasing ACTH concentrations over time; however, no significant effect of storing plasma in a serum tube was detected. Three horses diagnosed with PPID would have had a different classification if the samples had been kept at 20 °C for 24 or 48 hours.</p><p><strong>Conclusions: </strong>Time but not storage of plasma in serum tubes decreases the measured ACTH concentration in horses with PPID.</p><p><strong>Clinical relevance: </strong>Transferring plasma into serum tubes does not significantly alter the measured ACTH concentrations; however, time between collection and analysis might lead to erroneous diagnoses.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":""},"PeriodicalIF":1.3000,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"American journal of veterinary research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.2460/ajvr.24.11.0362","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/4/1 0:00:00","PubModel":"Print","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: Pituitary pars intermedia dysfunction (PPID) is diagnosed by measuring the ACTH concentrations. Due to the reported instability of ACTH, it is recommended to transfer centrifuged plasma into cryovials; however, in practice, cryovials are infrequently used, and serum (red-top) tubes are used instead. This study investigated whether this procedure affects ACTH concentrations and the diagnosis of PPID.
Methods: This was a cohort study. Blood was collected into EDTA tubes from 9 horses with PPID and 7 controls. After centrifugation, plasma was either aliquoted into a cryovial or into a serum tube. Samples were stored at 4 and 20 °C with ACTH concentrations measured using a chemiluminescent assay at 2, 24, and 48 hours after collection. Data were analyzed using a 2-way repeated measures ANOVA with P < .05 considered significant.
Results: There was a significant effect of time on ACTH concentrations in horses with PPID at 4 and 20 °C and in all horses at 20 °C with decreasing ACTH concentrations over time; however, no significant effect of storing plasma in a serum tube was detected. Three horses diagnosed with PPID would have had a different classification if the samples had been kept at 20 °C for 24 or 48 hours.
Conclusions: Time but not storage of plasma in serum tubes decreases the measured ACTH concentration in horses with PPID.
Clinical relevance: Transferring plasma into serum tubes does not significantly alter the measured ACTH concentrations; however, time between collection and analysis might lead to erroneous diagnoses.
期刊介绍：
The American Journal of Veterinary Research supports the collaborative exchange of information between researchers and clinicians by publishing novel research findings that bridge the gulf between basic research and clinical practice or that help to translate laboratory research and preclinical studies to the development of clinical trials and clinical practice. The journal welcomes submission of high-quality original studies and review articles in a wide range of scientific fields, including anatomy, anesthesiology, animal welfare, behavior, epidemiology, genetics, heredity, infectious disease, molecular biology, oncology, pharmacology, pathogenic mechanisms, physiology, surgery, theriogenology, toxicology, and vaccinology. Species of interest include production animals, companion animals, equids, exotic animals, birds, reptiles, and wild and marine animals. Reports of laboratory animal studies and studies involving the use of animals as experimental models of human diseases are considered only when the study results are of demonstrable benefit to the species used in the research or to another species of veterinary interest. Other fields of interest or animals species are not necessarily excluded from consideration, but such reports must focus on novel research findings. Submitted papers must make an original and substantial contribution to the veterinary medicine knowledge base; preliminary studies are not appropriate.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
