Impact of Paracoccus sp. EGY7 carotenoids on triple-negative breast cancer cells: invitro study.

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Karim Abdelazim, Ahmed Hussein, Sherine N Khattab, Shaymaa Essam El Feky, Nehad Noby
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Abstract

This study investigates the effect of Paracoccus sp. EGY7 carotenoids on the triple-negative breast cancer cell line (MDA-MB-231). The bacterial strain was isolated, and its carotenoids profile was analyzed via HPLC-DAD-MS. Cytotoxicity, migration tests and the expression of BAX and BCL-2 at the gene and protein levels were carried out to evaluate the therapeutic efficiency of the produced carotenoids. Molecular docking analysis estimated the binding affinity between zeaxanthin and BCL-2 protein. Chromatographic analysis revealed zeaxanthin as the major carotenoid (48.3%). The extract exhibited significant cytotoxicity against MDA-MB-231 cells with an IC50 of 1200 µg. It notably reduced cell migration, with wound closure percentages of 37.50% and 79.17% for the 600 µg group, while the percentages were 12.50% and 53.50% for the 1200 µg group, compared to 71.67% and 95.67% for the control at 24 and 48 h post-treatment, respectively. The extract induced apoptosis, as evidenced by significantly increased BAX/BCL-2 gene expression ratios at 600 and 1200 µg (p < 0.05). Western blotting showed increased BAX protein expression at 600 and 1200 µg compared to the control group (p < 0.001), and significantly lower BCL-2 protein expression (p = 0.000005 for 1200 µg and p = 0.0001 for 600 µg). Docking analysis indicated a strong affinity of zeaxanthin to BCL-2 (ΔG = -9.773241 kcal/mol) compared to obatoclax (ΔG = -7.419345 kcal/mol). Paracoccus sp. EGY7 carotenoids are a promising anticancer agent against MDA-MB-231 cells. They effectively promote apoptosis and prevent metastasis, crucial for disease advancement in cancer cells.

副球菌EGY7类胡萝卜素对三阴性乳腺癌细胞的影响:体外研究
本研究探讨了副球菌sp. EGY7类胡萝卜素对三阴性乳腺癌细胞株MDA-MB-231的影响。分离得到菌株,并通过HPLC-DAD-MS对其类胡萝卜素谱进行分析。通过细胞毒性、迁移试验以及BAX和BCL-2在基因和蛋白水平上的表达来评价所制备的类胡萝卜素的治疗效果。分子对接分析测定了玉米黄质与BCL-2蛋白的结合亲和力。色谱分析显示玉米黄质为主要的类胡萝卜素(48.3%)。提取物对MDA-MB-231细胞具有显著的细胞毒性,IC50为1200µg。处理后24和48 h, 600µg组伤口愈合率分别为37.50%和79.17%,1200µg组伤口愈合率分别为12.50%和53.50%,对照组分别为71.67%和95.67%。600µg和1200µg (p)时,BAX/BCL-2基因表达率显著升高
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来源期刊
AMB Express
AMB Express BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
7.20
自引率
2.70%
发文量
141
审稿时长
13 weeks
期刊介绍: AMB Express is a high quality journal that brings together research in the area of Applied and Industrial Microbiology with a particular interest in ''White Biotechnology'' and ''Red Biotechnology''. The emphasis is on processes employing microorganisms, eukaryotic cell cultures or enzymes for the biosynthesis, transformation and degradation of compounds. This includes fine and bulk chemicals, polymeric compounds and enzymes or other proteins. Downstream processes are also considered. Integrated processes combining biochemical and chemical processes are also published.
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