Sustainable One-Pot Electrochemical Approach for Entrapment of Multi-Enzymes and Biocompatible Redox Mediator

IF 3 4区 化学 Q2 CHEMISTRY, MULTIDISCIPLINARY
Dr. Hathaichanok Seelajaroen, Felix Mayr, Dr. Dominik Wielend, Dr. Munise Cobet, Dr. Christoph Ulbricht, Prof. Niyazi Serdar Sariciftci, Dr. Serpil Tekoglu
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引用次数: 0

Abstract

Enzyme immobilization is regarded as a key factor for their effective utilization in various fields such as biofuel production, wastewater treatment, and biosensors. Designing new electrodes with biocompatible support matrices is essential to improve the stability in bioelectrocatalysis. Modified carbon felt electrodes were prepared and tested as bioelectrocatalyst under mild conditions – aqueous media at room temperature and basically neutral pH. Co-immobilization of dehydrogenase enzymes and neutral red (NR) dye at carbon felt electrodes was successfully achieved during electropolymerization of pyrrole in a facile one-step approach. Neutral red was incorporated to operate as a redox mediator supporting efficient electron transfer to the enzymes’ active sites. Electrodes modified with alcohol dehydrogenase (ADH) have been employed to reduce acetaldehyde to ethanol in a chronoamperometic setting with Faradaic efficiency (FE) of up to 33 %. By incorporating the cascade reaction with three enzymes – ADH, formate dehydrogenase, and formaldehyde dehydrogenase – an electroreduction sequence could be established to produce methanol from CO2 reaching a FE of 10 %. The proposed approach shows good stability. Together with the simple implementation and application, this process is promising for employment in enzymatic electrocatalysis.

多酶包埋及生物相容性氧化还原介质的可持续一锅电化学方法。
酶固定化被认为是其在生物燃料生产、废水处理和生物传感器等各个领域有效利用的关键因素。设计具有生物相容性支撑基质的新型电极是提高生物电催化稳定性的必要条件。制备了改性碳毡电极,并在室温、ph基本为中性的水介质中作为生物电催化剂进行了测试。在电聚合吡咯过程中,成功地实现了脱氢酶和中性红染料在碳毡电极上的共固定化。中性红色被加入作为氧化还原介质,支持有效的电子转移到酶的活性位点。用乙醇脱氢酶(ADH)修饰的电极已被用于将乙醛还原为乙醇,其法拉第效率(FE)高达33%。通过与ADH、甲酸脱氢酶、甲醛脱氢酶等3种酶的级联反应,建立了以FE为10%的CO2为原料制备甲醇的电还原序列。该方法具有良好的稳定性。该工艺易于实现和应用,在酶催化电催化领域具有广阔的应用前景。
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来源期刊
ChemPlusChem
ChemPlusChem CHEMISTRY, MULTIDISCIPLINARY-
CiteScore
5.90
自引率
0.00%
发文量
200
审稿时长
1 months
期刊介绍: ChemPlusChem is a peer-reviewed, general chemistry journal that brings readers the very best in multidisciplinary research centering on chemistry. It is published on behalf of Chemistry Europe, an association of 16 European chemical societies. Fully comprehensive in its scope, ChemPlusChem publishes articles covering new results from at least two different aspects (subfields) of chemistry or one of chemistry and one of another scientific discipline (one chemistry topic plus another one, hence the title ChemPlusChem). All suitable submissions undergo balanced peer review by experts in the field to ensure the highest quality, originality, relevance, significance, and validity.
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