Early diagnosis of oral submucous fibrosis using salivary 8-OHDG and 8-Isoprostane.

Abstract

Oral submucous fibrosis (OSMF) is a condition that may be cancerous. The prognosis of OSMF is determined by a number of biomarkers, including 8-hydroxy 2' de-oxy-guanosine and 8-isoprostane. It is possible to assess the levels of 8-OHdG and 8-isoprostane in blood and saliva. Therefore, it is of interest to estimate salivary 8-OHdG and 8-isoprostane levels in order to diagnose oral submucous fibrosis. A sample size of 40 was divided into two groups with 20 samples in each, i.e., Group I - Healthy group (gutka consumers without any lesion) and Group II -Test (gutka consumers with OSMF). Samples of serum and saliva were taken from each group. Then samples were centrifuged for 15-20 minutes at 1000 RPM and 2-8°C. The resulting supernatant was pipetted out into labelled Eppendorf tubes in a volume of 1.5 ml, and it was then kept at 80°C. 8-OHdG and 8-isoprostane concentrations in various samples were determined using the ELISA technique. Serum's 8-OHdG content was considerably lower than saliva sample (P-value <0.05). The test group exhibited increased concentrations of 8-OHdG and 8-isoprostane in both saliva and serum samples when compared to the control group. 8-OHdG and 8-isoprostane can be utilised to diagnoses of OSMF.