Antibacterial Activity and Mechanisms of Magnesium-Doped Baghdadite Bioceramics for Orthopedic Implants

IF 4 Q2 ENGINEERING, BIOMEDICAL

Advanced Nanobiomed Research Pub Date : 2024-11-29 DOI:10.1002/anbr.202400119

Huu Ngoc Nguyen, Iman Roohani, Andrew Hayles, Zufu Lu, Jitraporn Vongsvivut, Krasimir Vasilev, Vi Khanh Truong, Hala Zreiqat

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Abstract

Baghdadite (BAG, Ca₃ZrSi₂O₉), a calcium silicate compound with zirconium incorporation, shows significant potential in medical implants. However, its susceptibility to infections poses a considerable challenge. To tackle this problem, doping biocompatible magnesium (Mg) into BAG to create Mg-BAG enhances antibacterial activity and prevents infection in orthopedic implants. Mg-BAG demonstrates effectiveness against Gram-positive Staphylococcus aureus and Gram-negative Pseudomonas aeruginosa. This study finds that the antibacterial activity of Mg-BAG is multifaced including causing the generation of reactive oxygen species (ROS) within cells and disrupting membrane potential, resulting in leakage of intracellular contents. The synchrotron macro attenuated total reflectance Fourier-transform infrared microspectroscopy shows the impact of Mg-BAG on bacteria, resulting in modifications to biomolecules such as lipids, protein structures, and the stability of nucleic acids. The combined effect of Mg ions (Mg²⁺) and intracellular ROS formation contributes to the disruption of biomolecules and bacterial cell death. Mg-BAG is a promising next-generation bioceramic offering innovative nonantibiotic solutions for preventing infection.

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来源期刊

Advanced Nanobiomed Research nanomedicine, bioengineering and biomaterials-

CiteScore

5.00

自引率

5.90%

发文量

审稿时长

21 weeks

期刊介绍： Advanced NanoBiomed Research will provide an Open Access home for cutting-edge nanomedicine, bioengineering and biomaterials research aimed at improving human health. The journal will capture a broad spectrum of research from increasingly multi- and interdisciplinary fields of the traditional areas of biomedicine, bioengineering and health-related materials science as well as precision and personalized medicine, drug delivery, and artificial intelligence-driven health science. The scope of Advanced NanoBiomed Research will cover the following key subject areas: ▪ Nanomedicine and nanotechnology, with applications in drug and gene delivery, diagnostics, theranostics, photothermal and photodynamic therapy and multimodal imaging. ▪ Biomaterials, including hydrogels, 2D materials, biopolymers, composites, biodegradable materials, biohybrids and biomimetics (such as artificial cells, exosomes and extracellular vesicles), as well as all organic and inorganic materials for biomedical applications. ▪ Biointerfaces, such as anti-microbial surfaces and coatings, as well as interfaces for cellular engineering, immunoengineering and 3D cell culture. ▪ Biofabrication including (bio)inks and technologies, towards generation of functional tissues and organs. ▪ Tissue engineering and regenerative medicine, including scaffolds and scaffold-free approaches, for bone, ligament, muscle, skin, neural, cardiac tissue engineering and tissue vascularization. ▪ Devices for healthcare applications, disease modelling and treatment, such as diagnostics, lab-on-a-chip, organs-on-a-chip, bioMEMS, bioelectronics, wearables, actuators, soft robotics, and intelligent drug delivery systems. with a strong focus on applications of these fields, from bench-to-bedside, for treatment of all diseases and disorders, such as infectious, autoimmune, cardiovascular and metabolic diseases, neurological disorders and cancer; including pharmacology and toxicology studies.