A Novel Effect of Allium tuncelianum Extract: Topical Application Improves Wound Healing in a Nasal Septal Perforation Rat Model

IF 1.6 4区医学 Q2 OTORHINOLARYNGOLOGY

Laryngoscope Investigative Otolaryngology Pub Date : 2025-02-08 DOI:10.1002/lio2.70095

Doğan Çakan, Zeliha Kübra Çakan, Gülderen Yılmaz, David Yeung, Gülnur Ekşi Bona

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Abstract

Objective

The objective of the current study was to investigate the influence of Allium tuncelianum (AT) extract on wound healing in a nasal septum perforation (NSP) model.

Methods

Twenty-two healthy male Sprague–Dawley rats were included in this study and separated into two groups. A standardized NSP was created in each subject. A 0.09% saline (control group) and the 25% AT solution (study group) were delivered intranasally daily for 14 days. At the end of the experiment, the subjects were sacrificed and the septa were excised for histopathological investigation. The macroscopic closure rate of NSP, intranasal pH, counts of acute inflammatory cells, eosinophil, fibroblast, and giant cell, epithelial regeneration and degeneration, vascularization, granulation formation, collagen density, cartilage regeneration, and degeneration were examined. The obtained data were analyzed statistically.

Results

The macroscopic closure rate (p = 0.006), the fibroblast number (p = 0.003), vascularization (p = 0.003), collagen density (p = 0.044), and granulation tissue amount (p = 0.022) were found to be significantly higher in AT group. However, the acute inflammatory cells count was significantly lower in AT group (p = 0.031) (p < 0.05).

Conclusion

The topically delivered AT extract may improve wound healing in an experimental NSP model. Consequently, the local application of AT might be promising to prevent the formation of NSP.

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葱提取物的新作用：局部应用促进鼻中隔穿孔大鼠模型的伤口愈合

目的探讨大蒜提取物对鼻中隔穿孔（NSP）模型创面愈合的影响。方法选取健康雄性Sprague-Dawley大鼠22只，随机分为两组。在每个科目中创建了标准化的NSP。每天经鼻给药0.09%生理盐水（对照组）和25% AT溶液（研究组），连续14天。实验结束时，处死受试者，切除间隔进行组织病理学检查。肉眼观察NSP闭合率、鼻内pH值、急性炎症细胞、嗜酸性粒细胞、成纤维细胞和巨细胞计数、上皮再生和变性、血管形成、肉芽形成、胶原密度、软骨再生和变性。对所得数据进行统计学分析。结果AT组的宏观闭合率（p = 0.006）、成纤维细胞数量（p = 0.003）、血管形成（p = 0.003）、胶原密度（p = 0.044）、肉芽组织数量（p = 0.022）均显著高于AT组。而AT组急性炎症细胞计数明显降低（p = 0.031）（p < 0.05）。结论外用AT提取物可促进实验性NSP模型创面愈合。因此，局部应用AT可能有望防止NSP的形成。

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