Quantitative Comparison of Different Parts of Tribulus terrestris L. at Different Harvest Times and Comparison of Antihypertensive Effects at the Same Harvest Time

IF 1.8 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Biomedical Chromatography Pub Date : 2025-02-07 DOI:10.1002/bmc.70005

Fei Li, De-Feng DU, Ming-Yue Chen, Xiao-Meng Gong, Hui-Min Su, Yi-Fan Wang, Lei Shi, Chao Zhang, Fei Guo

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Abstract

The whole plant of Tribulus terrestris (TT) is used to treat hypertension and cardio-cerebrovascular diseases. The harvest time has an important influence on the quality of medicinal plants. Therefore, it is necessary to study the effect of harvest time on the content of components in different parts of TT and compare the antihypertensive effect of different parts of TT at harvest time with a relatively high content of 14 components. Ultraviolet–visible spectrometry (UV–Vis spectrometry) was used to determine the total saponin content in T. terrestris fruit (TTF), T. terrestris stems (TTS), and T. terrestris leaves (TTL) at different harvest times. Within the studied harvest times, the total saponin content was higher in TTL than in TTF or TTS. Ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry analysis revealed significant differences in the spatial and temporal differences in the content of 14 components in the three plant parts. The multivariate statistical analysis identified six components as markers of different TT content in the different parts. The suitable TTF harvest period was from August 11 to September 1, while TTS and TTL were suitable for harvesting from July 21 to September 11. The treatment effects on blood pressure, serum indexes, and thoracic aorta morphology confirmed that the antihypertensive effects of TTL were better than those of TTF or TTS. The study emphasized the effect of harvest time on the content of components in different parts of TT and confirmed that TTS and TTL also had medicinal value.

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不同收获期刺蒺藜不同部位的定量比较和相同收获期抗高血压作用的比较

蒺藜（tritrus terrestris， TT）全株用于治疗高血压和心脑血管疾病。采收时间对药用植物的品质有重要影响。因此，有必要研究采收时间对黄芪不同部位成分含量的影响，比较14种成分含量较高的黄芪不同部位在采收时间的降压效果。采用紫外可见光谱法（UV-Vis）测定了不同采收期地参果实（TTF）、茎（TTS）和叶（TTL）中总皂苷的含量。在研究的收获期内，TTL处理的总皂苷含量高于TTF和TTS处理。超高效液相色谱-三重四极杆串联质谱分析显示，3种植物部位中14种成分含量存在显著的时空差异。通过多元统计分析，确定了6种成分作为不同部位TT含量的标志。TTF适宜采收期为8月11日~ 9月1日，TTS和TTL适宜采收期为7月21日~ 9月11日。对血压、血清指标及胸主动脉形态的治疗效果证实TTL的降压效果优于TTF或TTS。本研究强调了采收时间对黄芪不同部位成分含量的影响，证实了黄芪和黄芪也具有药用价值。

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来源期刊

Biomedical Chromatography 生物-分析化学

CiteScore

3.60

自引率

5.60%

发文量

268

审稿时长

2.3 months

期刊介绍： Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.