Therapeutic Potential of Artemisia campestris Essential Oil: Antioxidant, Anti-Inflammatory, and Anticancer Insights From In Silico Analysis

IF 1.8 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Biomedical Chromatography Pub Date : 2025-02-07 DOI:10.1002/bmc.70012

Inasse Cherfi, Mahboub Nasma, Gamil Gamal Hasan, Abir Benaissa, Youcef Benaissa, Salah Eddine Laouini, Abderrhmane Bouafia, Fahad Alharthi, Talha Bin Emran, Jewel Mallick

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Abstract

Artemisia campestris subsp. campestris (tuguft) is a medicinal plant traditionally used in Algerian medicine. This study investigates the chemical composition and bioactivity of its essential oil (ACEO). Gas chromatography–mass spectrometry (GC-MS) analysis identified key compounds, including linalyl acetate (2.92%), geranyl acetate (2.45%), and eucalyptol (1.38%). ACEO demonstrated significant antioxidant activity, with IC₅₀ values of 11.09 μg/mL (DPPH), 15.81 μg/mL (FRAP), and 22.70 μg/mL (β-carotene). It also enhanced peroxidase activity by 82.67 U/g. The anti-inflammatory effects were confirmed with an IC₅₀ of 18.87 μg/mL. Notably, in silico molecular docking revealed that 3-cyclopentyl-N-(2-(3,4-dimethoxyphenyl)ethyl) exhibits strong binding affinity to phosphoinositide 3-kinase gamma, a target for pancreatic cancer therapy, suggesting potential anticancer activity. These findings underscore the therapeutic potential of ACEO, highlighting its antioxidant, anti-inflammatory, and anticancer properties.

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野蒿精油的治疗潜力：从硅学分析中洞察抗氧化、抗炎和抗癌潜力

野蒿亚属。坎培斯特（tuguft）是阿尔及利亚传统医学中使用的药用植物。本研究对其精油的化学成分和生物活性进行了研究。气相色谱-质谱（GC-MS）分析鉴定出主要化合物，包括乙酸芳樟醇（2.92%）、乙酸香叶醇（2.45%）和桉树醇（1.38%）。ACEO具有显著的抗氧化活性，IC50值分别为11.09 μg/mL （DPPH）、15.81 μg/mL （FRAP）和22.70 μg/mL （β-胡萝卜素）。提高过氧化物酶活性82.67 U/g。抗炎作用证实，IC50为18.87 μg/mL。值得注意的是，在硅分子对接中发现，3-环戊基- n -（2-（3,4-二甲氧基苯基）乙基）与磷酸肌肽3-激酶γ具有很强的结合亲和力，这是胰腺癌治疗的靶点，表明可能具有抗癌活性。这些发现强调了ACEO的治疗潜力，突出了它的抗氧化、抗炎和抗癌特性。

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来源期刊

Biomedical Chromatography 生物-分析化学

CiteScore

3.60

自引率

5.60%

发文量

268

审稿时长

2.3 months

期刊介绍： Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.