Investigation of the Potential Treatment Effect of Botulinum Toxin Type A in Ovarian Torsion: Findings From the Rat Model

IF 2.5 3区医学 Q3 IMMUNOLOGY

American Journal of Reproductive Immunology Pub Date : 2025-02-08 DOI:10.1111/aji.70057

Çağlayan Ateş, Berna Dilbaz, Seval Yılmaz Ergani, Pelin Seçken, Caner Köse

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引用次数: 0

Abstract

Background

The aim of this study was to investigate the effects of botulinum toxin A on histological abnormalities, loss of ovarian reserve, and angiogenesis within the ovarian torsion–detorsion paradigm.

Methods

A total of 45 female Wistar albino rats were used for the study. The rats were randomly divided into five groups (n = 9 in each group): Control, Torsion, Torsion + Detorsion, Torsion + Detorsion + Botulinum toxin, and Botulinum toxin only. Follicular cell degeneration, vascular congestion, hemorrhage, inflammation, and vascular endothelial growth factor (VEGF) expression scores were compared histopathologically between the groups. Follicle numbers were compared. Anti-mullerian hormone levels were measured before and after torsion, detorsion, and treatments.

Results

Follicular cell degeneration, vascular congestion, hemorrhage, inflammation, and VEGF expression differed significantly between groups for each score (p < 0.001). The VEGF expression score was found to increase more in the torsion + detorsion + botulinum toxin group than in the torsion + detorsion group (2.67 ± 0.71 and 2.22 ± 0.67, respectively). There was a significant difference between the groups in secondary follicle counts (p = 0.04). The groups showed statistically significant differences in their anti-mullerian hormone levels (p < 0.001).

Conclusion

This initial investigation of the effects of botulinum toxin on ovarian torsion (OT) has shown that botulinum toxin type A has the potential to be a useful adjunct treatment for fertility preservation. Our results proved the ovarian protective effect of botulinum toxin type A in rats with OT. Therefore, botulinum toxin type A may be a potential preventive agent against ovarian damage caused by torsion in the future.

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A型肉毒毒素对卵巢扭转潜在治疗作用的研究：来自大鼠模型的发现

本研究的目的是探讨肉毒杆菌毒素A对卵巢扭转-扭转模式下的组织学异常、卵巢储备丧失和血管生成的影响。方法选用45只雌性Wistar白化大鼠进行实验研究。将大鼠随机分为5组（每组n = 9）：对照组、扭转组、扭转+扭转组、扭转+扭转+肉毒杆菌毒素组和仅肉毒杆菌毒素组。比较两组间滤泡细胞变性、血管充血、出血、炎症及血管内皮生长因子（VEGF）表达评分。比较卵泡数。在扭转、扭曲和治疗前后测定抗苗勒管激素水平。结果各组间滤泡细胞变性、血管充血、出血、炎症及VEGF表达差异有统计学意义(p <；0.001)。VEGF表达评分在扭转+扭转+肉毒毒素组明显高于扭转+扭转组（分别为2.67±0.71和2.22±0.67）。两组间继发卵泡计数差异有统计学意义（p = 0.04）。两组抗苗勒管激素水平差异有统计学意义(p <；0.001)。结论对A型肉毒毒素对卵巢扭转（OT）影响的初步研究表明，A型肉毒毒素有可能作为一种有效的辅助治疗手段来保留生育能力。我们的结果证实了A型肉毒毒素对OT大鼠卵巢的保护作用。因此，A型肉毒杆菌毒素可能是一种潜在的预防卵巢扭转损伤的药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

American Journal of Reproductive Immunology 医学-免疫学

CiteScore

6.20

自引率

5.60%

发文量

314

审稿时长

2 months

期刊介绍： The American Journal of Reproductive Immunology is an international journal devoted to the presentation of current information in all areas relating to Reproductive Immunology. The journal is directed toward both the basic scientist and the clinician, covering the whole process of reproduction as affected by immunological processes. The journal covers a variety of subspecialty topics, including fertility immunology, pregnancy immunology, immunogenetics, mucosal immunology, immunocontraception, endometriosis, abortion, tumor immunology of the reproductive tract, autoantibodies, infectious disease of the reproductive tract, and technical news.