Advancements of the Vibrio coralliilyticus eDNA detection based on Co-Fe PBA-assisted biosensors for the rapid coral-disease warning

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2025-02-06 DOI:10.1016/j.bej.2025.109662

Yibo Zhang , Hongjie Liu , Shaopeng Wang , Hao Fu , Yuanyu Xie , Chaoxin Zhang , Man Zhang , Jie Lu , Liwei Wang , Kefu Yu

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引用次数: 0

Abstract

Vibrio coralliilyticus (V. coralliilyticus) can lead to severe coral diseases and is one of the main causes of coral bleaching. However, there is still a lack of convenient, and non-invasive detection methods for V. coralliilyticus, which brings inconvenience to coral health management. Here, a portable electrochemical biosensor was developed based on environmental DNA (eDNA) and cobalt-iron Prussian Blue Analogue (Co-Fe PBA) electrode material for detecting V. coralliilyticus. Notably, this sensor identified and quantified V. coralliilyticus eDNA in water through specific DNA probes, overcoming the previously reported complex and destructive pretreatment processes. Additionally, thanks to the high specific surface area of Co-Fe PBA, which provided abundant sites for probe anchoring, the constructed portable biosensor exhibited excellent detection performances. Specifically, the detection limit was as low as 19.0 fM, and the linear range was from 100 fM to 100 nM. In subsequent coral infection experiments, the eDNA of V. coralliilyticus increased on the eve of coral bleaching. Based on this change, the occurrence of coral bleaching was successfully predicted. Therefore, this portable biosensor is suitable for dynamic monitoring of V. coralliilyticus, helping to reveal the potential risks to coral health and providing a reference for ecological decision-making for coral reef protection.

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.