An engineered Yarrowia lipolytica with rapid growth and efficient lipid utilization

IF 4.4 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Synthetic and Systems Biotechnology Pub Date : 2025-01-26 DOI:10.1016/j.synbio.2025.01.007

Tianyu Dong , Yujie Shu , Ying Wang , Mingdong Yao , Wenhai Xiao

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Abstract

Yarrowia lipolytica, a safe yeast, efficiently metabolizes lipids for the production of food additives and agricultural products. Boosting its growth and lipid utilization capabilities is crucial to enhancing the overall efficiency Y. lipolytica. Herein, an integrated strategy was implemented to enhance lipid uptake, accumulation and metabolism and systematically promote the growth and lipid utilization of the commonly used Y. lipolytica Po1f strain. The engineered strain had a specific growth rate of 0.32 h⁻¹ and a lipid content of 67.66 % (g/g DCW), which were 54 % and 26 % greater than those of the original strain. β-Carotene was used to verify the production of lipophilic natural compounds, and the highest yield was obtained 48 h earlier using the engineered strain compared to the original strain when consuming same carbon source. These findings show promise in using engineered Y. lipolytica for rapid growth and improved lipid utilization to boost efficiency of lipophilic product production.

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具有快速生长和高效脂质利用的工程解脂耶氏菌

脂溶耶氏酵母是一种安全的酵母，它能有效地代谢脂质，用于生产食品添加剂和农产品。提高其生长和脂质利用能力是提高溶脂芽孢杆菌整体效率的关键。本研究通过提高脂质吸收、积累和代谢的综合策略，系统地促进常用的聚脂Y. polytica Po1f菌株的生长和脂质利用。工程菌株的比生长速率为0.32 h−1，脂质含量为67.66% (g/g DCW)，分别比原菌株高54%和26%。用β-胡萝卜素验证了亲脂性天然化合物的产量，在消耗相同碳源的情况下，工程菌株比原始菌株提前48 h产量最高。这些发现表明，利用工程解脂芽孢杆菌快速生长和改善脂质利用，以提高亲脂产物的生产效率是有希望的。

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来源期刊

Synthetic and Systems Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

6.90

自引率

12.50%

发文量

审稿时长

67 days

期刊介绍： Synthetic and Systems Biotechnology aims to promote the communication of original research in synthetic and systems biology, with strong emphasis on applications towards biotechnology. This journal is a quarterly peer-reviewed journal led by Editor-in-Chief Lixin Zhang. The journal publishes high-quality research; focusing on integrative approaches to enable the understanding and design of biological systems, and research to develop the application of systems and synthetic biology to natural systems. This journal will publish Articles, Short notes, Methods, Mini Reviews, Commentary and Conference reviews.