Novel aquaculture wastewater treatment by an efficient nitrogen-removing bacterium capable of simultaneous nitrification and denitrification

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2025-02-05 DOI:10.1016/j.bej.2025.109663

Yuhang Song , Lingmin Zhao , Lixing Huang , Yingxue Qin , Jiaonan Zhang , Jiaoling Zhang , Qingpi Yan

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Abstract

Nitrogen pollution from aquaculture effluent poses a severe threat to aquatic ecosystems, necessitating efficient treatment solutions. In this study, a highly effective nitrogen-removing bacterium, designated SA20, was isolated from an aquaculture wastewater treatment system and identified as Paracoccus sp. Safety evaluations confirmed its non-hemolytic nature and broad antibiotic sensitivity, indicating its potential for safe application. Under optimized conditions (C/N ratio 16, 30 ℃, 140 rpm), the strain demonstrated complete removal of ammonium and nitrite, with over 95 % nitrate removal within 24 h. Nitrogen balance analysis revealed that approximately 70–80 % of total nitrogen was converted to gaseous form through denitrification, while 20–30 % was assimilated into biomass. When applied to actual aquaculture wastewater, SA20 achieved significant removal of various nitrogen forms, with total nitrogen removal exceeding 65 %. These findings demonstrate the potential of strain SA20 for practical application in aquaculture wastewater treatment, offering a promising biotechnology solution for sustainable aquaculture development.

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新型水产养殖废水处理的高效脱氮细菌能够同时硝化和反硝化

水产养殖废水中的氮污染对水生生态系统构成严重威胁，需要有效的处理方案。本研究从水产养殖废水处理系统中分离出一种高效脱氮细菌，命名为SA20，鉴定为副球菌sp.安全性评价证实其非溶血性和广泛的抗生素敏感性，表明其具有安全应用的潜力。在优化条件（C/N比16、30 ℃、140 rpm）下，菌株对铵态氮和亚硝酸盐的去除率达到100%，24 h内硝酸盐去除率达到95% %以上。氮平衡分析表明，约70-80 %的总氮通过反硝化转化为气态形式，而20-30 %的总氮被同化为生物质。应用于实际养殖废水中，SA20对各种形态氮的去除效果显著，总氮去除率超过65% %。这些发现表明菌株SA20在水产养殖废水处理中具有实际应用潜力，为水产养殖可持续发展提供了一种有前景的生物技术解决方案。

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.