A procedure for total protein determination with special application to allergenic extract standardization

Journal of biological standardization Pub Date : 1988-01-01 DOI:10.1016/0092-1157(88)90010-8

Paul G. Richman , David S. Cissel

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引用次数: 16

Abstract

A method for total protein determination of allergenic extracts has been developed and evaluated. Samples were hydrolyzed with 5 m NaOH followed by colorimetric determination with ninhydrin of the released amino acids using bovine serum albumin as the standard. The entire procedure was carried out in disposable plastic tubes. Substances (glycerol, phenol and mannitol) commonly present in allergenic extracts manufactured for human use did not affect the assay results. Analyses of four different pollen extracts by the method gave good agreement with amino acid analyses. Other methods of analysis (total N, protein N unit assay, Lowry) gave more variable results compared with amino acid analysis. Analysis of the total protein content of 53 different lots of allergenic extracts gave narrow ranges of values for each species. Standardized mite extracts analyzed for total protein by US FDA-licensed manufacturers using this assay showed a good correlation of biological activity with total protein.

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过敏原提取物标准化中总蛋白的测定方法

建立了一种测定致敏提取物总蛋白的方法并进行了评价。样品用5 m氢氧化钠水解，以牛血清白蛋白为标准，用茚三酮比色法测定所释放的氨基酸。整个过程是在一次性塑料管中进行的。通常存在于人类使用的致敏提取物中的物质(甘油、苯酚和甘露醇)不影响检测结果。对四种不同花粉提取物的分析结果与氨基酸分析结果吻合较好。与氨基酸分析相比，其他分析方法(全N、蛋白N单位分析法、Lowry法)的结果更加多变。对53种不同批次的致敏提取物的总蛋白质含量进行分析，得出每个物种的值范围很窄。美国fda许可的制造商使用该方法对标准化的螨提取物进行了总蛋白分析，结果表明其生物活性与总蛋白具有良好的相关性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of biological standardization

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