Unlocking the full potential of plant cell-based production for valuable proteins: Challenges and innovative strategies

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances Pub Date : 2025-02-04 DOI:10.1016/j.biotechadv.2025.108526

Jianfeng Xu , Paula PerezSanchez , Shekoofeh Sadravi

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引用次数: 0

Abstract

Plant cell-based bioproduction systems offer a promising platform for the sustainable production of valuable proteins as they provide distinctive advantages over mammalian cell culture and whole plant cultivation. However, significant technical challenges remain, including low productivity, altered efficacy of plant-derived proteins, along with issues in culture process development, such as cell clumping, genetic instability, and difficulties with cryopreservation. To date, the full production potential of this platform remains largely untapped. This review addresses these critical challenges and proposes innovative strategies to unlock the full potential of the production platform. Rather than simply revisiting past advancements or summarizing current progress, it proposes forward-thinking solutions with a particular emphasis on cellular engineering. Key strategies include designing novel protein partners to enhance recombinant protein accumulation and functionality, employing precise gene integration techniques in genome to enhance transgene transcription, implementing cutting-edge methods for screening and maintaining elite cell lines to mitigate genetic instability, and leveraging genome editing tools for cellular engineering to develop new plant cell lines optimized for bioproduction. A key focus is on cell wall engineering to develop cellulose- or pectin-deficient cell lines, facilitating modifications to the morphology of existing plant cell lines. By exploring these innovative approaches, this review aims to foster innovative thinking and inspire future research in plant cell-based bioproduction.

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来源期刊

Biotechnology advances 工程技术-生物工程与应用微生物

CiteScore

25.50

自引率

2.50%

发文量

167

审稿时长

37 days

期刊介绍： Biotechnology Advances is a comprehensive review journal that covers all aspects of the multidisciplinary field of biotechnology. The journal focuses on biotechnology principles and their applications in various industries, agriculture, medicine, environmental concerns, and regulatory issues. It publishes authoritative articles that highlight current developments and future trends in the field of biotechnology. The journal invites submissions of manuscripts that are relevant and appropriate. It targets a wide audience, including scientists, engineers, students, instructors, researchers, practitioners, managers, governments, and other stakeholders in the field. Additionally, special issues are published based on selected presentations from recent relevant conferences in collaboration with the organizations hosting those conferences.