Nanopore characterization of DNA–COR–DNA duplex and the sensitive detection of coralyne

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-02-06 DOI:10.1016/j.snb.2025.137407

Ting Li , Shaojiao Song , Wei Lu , Yu Cao , Xin Zheng , Qiuyue Zhao , Hailong Wang

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引用次数: 0

Abstract

Owing to the high selectivity and binding affinity of coralyne (COR) with deoxyribonucleic acid (DNA)/ribonucleic acid, COR has been confirmed as an effective anticancer drug. However, a direct and sensitive detection assay for a DNA–COR–DNA structure with a portable instrument has not been reported because of the lack of a high-resolution sensor that can identify DNA–COR interaction at a single-molecule level. Here, specific Types I and II signals generated by a DNA–COR–DNA duplex were observed using a versatile α-hemolysin nanopore sensor. The Type I signal featuring a unique current pattern was preferentially selected via DNA sequence design and voltage control. Subsequently, the rapid and sensitive detection of COR was established with a detection limit of 0.1 nM. The pH environment, observed to influence the binding of the DNA–COR–DNA duplex, was significant for drug release controlling during targeted delivery process. Similar alkaloids were tested, and the nanopore sensor exhibited a high selectivity for COR. The results demonstrate a potential platform for investigating DNA-COR interaction and could be applied in the drug therapy and pharmacodynamic studies of COR.

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.