Fast In Vivo Two-Photon Fluorescence Imaging via Lateral and Axial Resolution Restoration With Self-Supervised Learning

IF 2 3区 物理与天体物理 Q3 BIOCHEMICAL RESEARCH METHODS
Zhengyuan Pan, Man Lei, Hongen Liao, Bobo Gu
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引用次数: 0

Abstract

Two-photon fluorescence (TPF) imaging opens a new avenue to achieve high resolution at extended penetration depths. However, it is difficult for conventional TPF imaging systems to simultaneously achieve high resolution and speed. In this work, we develop an innovative deep learning framework of Lateral and Axial Resolution Restoration (LARR) to break the contradiction between imaging resolution and speed. LARR employs a self-supervised training scheme to computationally restore the sparsely sampled TPF images to resolution isotropic images by 4-fold axial and 16-fold lateral resolution enhancement. The simulation studies and experimental results demonstrate the excellent performance of LARR to preserve fine structural features with improved signal-to-noise ratio and structure similarity index. Moreover, the TPF imaging system with the LARR is able to achieve 60-fold improved imaging speed and comparable resolution as compared with the conventional TPF system. The outstanding performance makes LARR a potential tool for fast TPF imaging with high resolution.

Abstract Image

快速体内双光子荧光成像通过横向和轴向分辨率恢复与自监督学习。
双光子荧光成像为实现大穿透深度下的高分辨率成像开辟了一条新途径。然而,传统的TPF成像系统很难同时实现高分辨率和高速度。在这项工作中,我们开发了一个创新的横向和轴向分辨率恢复(LARR)深度学习框架,以打破成像分辨率和速度之间的矛盾。LARR采用自监督训练方案,通过4倍轴向和16倍横向分辨率增强,计算恢复稀疏采样的TPF图像到分辨率各向同性图像。仿真研究和实验结果表明,LARR算法在保持精细结构特征的同时,提高了信噪比和结构相似度。此外,与传统的TPF系统相比,带有LARR的TPF成像系统能够实现60倍的成像速度和相当的分辨率。出色的性能使LARR成为高分辨率快速TPF成像的潜在工具。
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来源期刊
Journal of Biophotonics
Journal of Biophotonics 生物-生化研究方法
CiteScore
5.70
自引率
7.10%
发文量
248
审稿时长
1 months
期刊介绍: The first international journal dedicated to publishing reviews and original articles from this exciting field, the Journal of Biophotonics covers the broad range of research on interactions between light and biological material. The journal offers a platform where the physicist communicates with the biologist and where the clinical practitioner learns about the latest tools for the diagnosis of diseases. As such, the journal is highly interdisciplinary, publishing cutting edge research in the fields of life sciences, medicine, physics, chemistry, and engineering. The coverage extends from fundamental research to specific developments, while also including the latest applications.
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