{"title":"Fast In Vivo Two-Photon Fluorescence Imaging via Lateral and Axial Resolution Restoration With Self-Supervised Learning","authors":"Zhengyuan Pan, Man Lei, Hongen Liao, Bobo Gu","doi":"10.1002/jbio.202400489","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Two-photon fluorescence (TPF) imaging opens a new avenue to achieve high resolution at extended penetration depths. However, it is difficult for conventional TPF imaging systems to simultaneously achieve high resolution and speed. In this work, we develop an innovative deep learning framework of Lateral and Axial Resolution Restoration (LARR) to break the contradiction between imaging resolution and speed. LARR employs a self-supervised training scheme to computationally restore the sparsely sampled TPF images to resolution isotropic images by 4-fold axial and 16-fold lateral resolution enhancement. The simulation studies and experimental results demonstrate the excellent performance of LARR to preserve fine structural features with improved signal-to-noise ratio and structure similarity index. Moreover, the TPF imaging system with the LARR is able to achieve 60-fold improved imaging speed and comparable resolution as compared with the conventional TPF system. The outstanding performance makes LARR a potential tool for fast TPF imaging with high resolution.</p>\n </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 4","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Biophotonics","FirstCategoryId":"101","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jbio.202400489","RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
Two-photon fluorescence (TPF) imaging opens a new avenue to achieve high resolution at extended penetration depths. However, it is difficult for conventional TPF imaging systems to simultaneously achieve high resolution and speed. In this work, we develop an innovative deep learning framework of Lateral and Axial Resolution Restoration (LARR) to break the contradiction between imaging resolution and speed. LARR employs a self-supervised training scheme to computationally restore the sparsely sampled TPF images to resolution isotropic images by 4-fold axial and 16-fold lateral resolution enhancement. The simulation studies and experimental results demonstrate the excellent performance of LARR to preserve fine structural features with improved signal-to-noise ratio and structure similarity index. Moreover, the TPF imaging system with the LARR is able to achieve 60-fold improved imaging speed and comparable resolution as compared with the conventional TPF system. The outstanding performance makes LARR a potential tool for fast TPF imaging with high resolution.
期刊介绍:
The first international journal dedicated to publishing reviews and original articles from this exciting field, the Journal of Biophotonics covers the broad range of research on interactions between light and biological material. The journal offers a platform where the physicist communicates with the biologist and where the clinical practitioner learns about the latest tools for the diagnosis of diseases. As such, the journal is highly interdisciplinary, publishing cutting edge research in the fields of life sciences, medicine, physics, chemistry, and engineering. The coverage extends from fundamental research to specific developments, while also including the latest applications.