Kiminobu Sato, Mengya Mark, Xiaochuan Xu, Bernard Rousseau
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引用次数: 0
Abstract
Objectives: The comparative fine structures of the rabbit vocal fold (VF) were investigated using the technique combined with correlative light and electron microscopy (CLEM) and transmission electron microscopy (TEM) to elucidate the functional mechanisms.
Methods: Seven normal New Zealand White Rabbit vocal folds were examined using CLEM technique and TEM to investigate their fine structures.
Results: In contrast to the human VF, the rabbit VF lacked the macula flava and vocal ligament. However, the distribution of fibrous components of the lamina propria (LP) of the rabbit VF was similar to the human VF. Spindle-shaped and oval-shaped cells were identified in the LP of the membranous portion of the rabbit VF using CLEM. Furthermore, amorphous materials were produced from vesicles positioned at the periphery of fibroblast cytoplasm. Unlike the human macula flava, stellate cells containing lipid droplets in the cytoplasm were absent in the rabbit VF.
Conclusion: The potential utilization of the CLEM technique facilitates the observation of these fine structures in other species commonly employed in animal experiments. Rabbits are considered a suitable species for animal experiments due to the characteristics of the fibrous components of the VF, but it is crucial for researchers to understand the structural and functional distinctions of VF in other species utilized for animal experiments. As the CLEM is a useful technique to evaluate the fine structures of the VF across small species, it is expected to be applied in animal experiments in future studies.
期刊介绍：
The Laryngoscope has been the leading source of information on advances in the diagnosis and treatment of head and neck disorders since 1890. The Laryngoscope is the first choice among otolaryngologists for publication of their important findings and techniques. Each monthly issue of The Laryngoscope features peer-reviewed medical, clinical, and research contributions in general otolaryngology, allergy/rhinology, otology/neurotology, laryngology/bronchoesophagology, head and neck surgery, sleep medicine, pediatric otolaryngology, facial plastics and reconstructive surgery, oncology, and communicative disorders. Contributions include papers and posters presented at the Annual and Section Meetings of the Triological Society, as well as independent papers, "How I Do It", "Triological Best Practice" articles, and contemporary reviews. Theses authored by the Triological Society’s new Fellows as well as papers presented at meetings of the American Laryngological Association are published in The Laryngoscope.
• Broncho-esophagology
• Communicative disorders
• Head and neck surgery
• Plastic and reconstructive facial surgery
• Oncology
• Speech and hearing defects
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