In vitro micropropagation protocols for two endangered Dianthus species - via in vitro culture for conservation and recultivation purposes.

IF 4.7 2区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Plant Methods Pub Date : 2025-02-05 DOI:10.1186/s13007-025-01335-2

Dóra Farkas, Judit Csabai, Angéla Kolesnyk, Pál Szarvas, Judit Dobránszki

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引用次数: 0

Abstract

Background: D. giganteiformis subsp. pontederae and D. superbus subsp. superbus are protected or critically endangered species in several European regions; therefore, developing an efficient in vitro micropropagation protocol is essential for germplasm conservation and recultivation purposes.

Results: After germination, one-nodal segments of both species were transferred onto several MS media supplemented with 3% sucrose and different types of cytokinins (at a concentration of 4.5 µM) alongside 0.54 µM 1-naphthaleneacetic acid (NAA) for the multiplication phase for 3 weeks. The shoot clusters were subsequently transferred onto elongation medium (plant growth regulator-free MS medium) for 3 weeks. Individual shoots separated from the shoot clusters were cultured on MS medium supplemented with 0.54 µM NAA and 2% sucrose for 3 weeks for rooting. Taking into account the effects and after-effects of cytokinins, we found that the most suitable cytokinin for D. giganteiformis subsp. pontederae was N-(2-isopentenyl)-adenine (2-iP), while for D. superbus subsp. superbus it was meta-topolin (mT).

Conclusions: In vitro micropropagation methods were developed for two endangered Dianthus species (D. giganteiformis subsp. pontederae and D. superbus subsp. superbus) by determining the optimal type of cytokinin to be used during the multiplication phase. The protocols are designed to produce large quantities of propagation material for recultivation, educational, and research purposes within three months.

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两种濒危石竹的离体微繁殖方法——保护和再培养的离体培养。

背景：D. giganteiformis subsp。蜈蚣科和蜈蚣亚科。在几个欧洲地区，超级大黄蜂是受保护的或极度濒危的物种；因此，开发一种高效的离体微繁技术对种质资源保护和再培养具有重要意义。结果：发芽后，将两种植物的单节片段转移到添加3%蔗糖和不同类型细胞分裂素（浓度为4.5µM）的MS培养基上（浓度为0.54µM 1-萘乙酸（NAA）），进行3周的增殖。随后将茎簇转移到延伸培养基（不含植物生长调节剂的MS培养基）上3周。从芽簇中分离出单株芽，在添加0.54µM NAA和2%蔗糖的MS培养基上培养3周生根。综合考虑细胞分裂素的作用和后效，我们发现最适合巨形拟虫亚种的细胞分裂素。pontederae为N-（2-异戊烯基）-腺嘌呤（2-iP）；它是元topolin （mT）。结论：建立了两种濒危石竹（d.d . giganteiformis subsp）的体外微繁方法。蜈蚣科和蜈蚣亚科。通过确定在增殖阶段使用的细胞分裂素的最佳类型。该方案旨在在三个月内生产大量繁殖材料用于再培养，教育和研究目的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Methods 生物-植物科学

CiteScore

9.20

自引率

3.90%

发文量

121

审稿时长

2 months

期刊介绍： Plant Methods is an open access, peer-reviewed, online journal for the plant research community that encompasses all aspects of technological innovation in the plant sciences. There is no doubt that we have entered an exciting new era in plant biology. The completion of the Arabidopsis genome sequence, and the rapid progress being made in other plant genomics projects are providing unparalleled opportunities for progress in all areas of plant science. Nevertheless, enormous challenges lie ahead if we are to understand the function of every gene in the genome, and how the individual parts work together to make the whole organism. Achieving these goals will require an unprecedented collaborative effort, combining high-throughput, system-wide technologies with more focused approaches that integrate traditional disciplines such as cell biology, biochemistry and molecular genetics. Technological innovation is probably the most important catalyst for progress in any scientific discipline. Plant Methods’ goal is to stimulate the development and adoption of new and improved techniques and research tools and, where appropriate, to promote consistency of methodologies for better integration of data from different laboratories.