Synergistic effects of zP-1 phage and ampicillin against methicillin-resistant Staphylococcus aureus isolated from hospital staff.

IF 1.7 4区医学 Q2 MEDICINE, GENERAL & INTERNAL

Irish Journal of Medical Science Pub Date : 2025-02-06 DOI:10.1007/s11845-025-03898-4

Zain Mehmood, Rabia Kanwar, Kaleem Ullah, Saqib Ali, Muhammad Aamir Aslam, Abdul Qadeer, Haiwad Gul, Mohamed Hassanein Mahmoud

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Abstract

Background: Methicillin-resistant Staphylococcus aureus (MRSA) strains are a major cause of hospital-acquired infections, exacerbated by increasing antibiotic resistance.

Aim: This study aimed to isolate bacteriophages targeting MRSA and evaluate their antimicrobial activity in combination with antibiotics.

Methods: Nasal samples from hospital staff (n = 50) were used to isolate MRSA strains, and sewage samples were processed for phage isolation using the double agar overlay technique. The microtitration plate method evaluated the synergistic effects of isolated phages and antibiotics.

Results: Out of 50 samples, 11 MRSA strains were positive, showing high resistance to multiple antibiotics, including oxacillin (95%), and vancomycin (85%). Phage zP-1, belonging to the Myoviridae family, exhibited > 90% lytic activity, stable across temperatures (20-50 °C) and pH (6-8). A statistically significant synergistic effect was observed at a MIC of 0.137 μg/ml for gentamicin (p-value 0.02).

Conclusion: These findings suggest that phage-antibiotic synergy may offer a promising strategy to combat MRSA infections, warranting further in vivo studies to assess its clinical potential.

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来源期刊

Irish Journal of Medical Science 医学-医学：内科

CiteScore

3.70

自引率

4.80%

发文量

357

审稿时长

4-8 weeks

期刊介绍： The Irish Journal of Medical Science is the official organ of the Royal Academy of Medicine in Ireland. Established in 1832, this quarterly journal is a contribution to medical science and an ideal forum for the younger medical/scientific professional to enter world literature and an ideal launching platform now, as in the past, for many a young research worker. The primary role of both the Academy and IJMS is that of providing a forum for the exchange of scientific information and to promote academic discussion, so essential to scientific progress.