Synergistic effects of zP-1 phage and ampicillin against methicillin-resistant Staphylococcus aureus isolated from hospital staff.

IF 1.7 4区医学 Q2 MEDICINE, GENERAL & INTERNAL

Irish Journal of Medical Science Pub Date : 2025-02-06 DOI:10.1007/s11845-025-03898-4

Zain Mehmood, Rabia Kanwar, Kaleem Ullah, Saqib Ali, Muhammad Aamir Aslam, Abdul Qadeer, Haiwad Gul, Mohamed Hassanein Mahmoud

{"title":"Synergistic effects of zP-1 phage and ampicillin against methicillin-resistant Staphylococcus aureus isolated from hospital staff.","authors":"Zain Mehmood, Rabia Kanwar, Kaleem Ullah, Saqib Ali, Muhammad Aamir Aslam, Abdul Qadeer, Haiwad Gul, Mohamed Hassanein Mahmoud","doi":"10.1007/s11845-025-03898-4","DOIUrl":null,"url":null,"abstract":"Background: Methicillin-resistant Staphylococcus aureus (MRSA) strains are a major cause of hospital-acquired infections, exacerbated by increasing antibiotic resistance.Aim: This study aimed to isolate bacteriophages targeting MRSA and evaluate their antimicrobial activity in combination with antibiotics.Methods: Nasal samples from hospital staff (n = 50) were used to isolate MRSA strains, and sewage samples were processed for phage isolation using the double agar overlay technique. The microtitration plate method evaluated the synergistic effects of isolated phages and antibiotics.Results: Out of 50 samples, 11 MRSA strains were positive, showing high resistance to multiple antibiotics, including oxacillin (95%), and vancomycin (85%). Phage zP-1, belonging to the Myoviridae family, exhibited > 90% lytic activity, stable across temperatures (20-50 °C) and pH (6-8). A statistically significant synergistic effect was observed at a MIC of 0.137 μg/ml for gentamicin (p-value 0.02).Conclusion: These findings suggest that phage-antibiotic synergy may offer a promising strategy to combat MRSA infections, warranting further in vivo studies to assess its clinical potential.","PeriodicalId":14507,"journal":{"name":"Irish Journal of Medical Science","volume":" ","pages":""},"PeriodicalIF":1.7000,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Irish Journal of Medical Science","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s11845-025-03898-4","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Methicillin-resistant Staphylococcus aureus (MRSA) strains are a major cause of hospital-acquired infections, exacerbated by increasing antibiotic resistance.

Aim: This study aimed to isolate bacteriophages targeting MRSA and evaluate their antimicrobial activity in combination with antibiotics.

Methods: Nasal samples from hospital staff (n = 50) were used to isolate MRSA strains, and sewage samples were processed for phage isolation using the double agar overlay technique. The microtitration plate method evaluated the synergistic effects of isolated phages and antibiotics.

Results: Out of 50 samples, 11 MRSA strains were positive, showing high resistance to multiple antibiotics, including oxacillin (95%), and vancomycin (85%). Phage zP-1, belonging to the Myoviridae family, exhibited > 90% lytic activity, stable across temperatures (20-50 °C) and pH (6-8). A statistically significant synergistic effect was observed at a MIC of 0.137 μg/ml for gentamicin (p-value 0.02).

Conclusion: These findings suggest that phage-antibiotic synergy may offer a promising strategy to combat MRSA infections, warranting further in vivo studies to assess its clinical potential.

查看原文本刊更多论文

zP-1噬菌体与氨苄西林对医院工作人员耐甲氧西林金黄色葡萄球菌的协同作用

背景：耐甲氧西林金黄色葡萄球菌（MRSA）菌株是医院获得性感染的主要原因，随着抗生素耐药性的增加而加剧。目的：分离靶向MRSA的噬菌体，并评价其与抗生素联合抗菌活性。方法：采用医院工作人员鼻腔标本（50例）分离MRSA菌株，污水标本采用双琼脂覆盖法分离噬菌体。微滴平板法评价分离噬菌体与抗生素的协同作用。结果：50份样本中，11株MRSA阳性，对多种抗生素耐药，包括oxacillin（95%）和万古霉素（85%）。噬菌体zP-1属于肌病毒科，具有90%的裂解活性，在温度（20 ~ 50℃）和pH（6 ~ 8）范围内稳定。庆大霉素MIC为0.137 μg/ml时，增效效果具有统计学意义（p值为0.02）。结论：这些发现表明，噬菌体-抗生素协同作用可能是对抗MRSA感染的一种有希望的策略，需要进一步的体内研究来评估其临床潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Irish Journal of Medical Science 医学-医学：内科

CiteScore

3.70

自引率

4.80%

发文量

357

审稿时长

4-8 weeks

期刊介绍： The Irish Journal of Medical Science is the official organ of the Royal Academy of Medicine in Ireland. Established in 1832, this quarterly journal is a contribution to medical science and an ideal forum for the younger medical/scientific professional to enter world literature and an ideal launching platform now, as in the past, for many a young research worker. The primary role of both the Academy and IJMS is that of providing a forum for the exchange of scientific information and to promote academic discussion, so essential to scientific progress.