Turning over new ideas in human skeletal muscle proteostasis: What do we know and where to from here?

IF 2.6 4区医学 Q2 PHYSIOLOGY

Experimental Physiology Pub Date : 2025-02-05 DOI:10.1113/EP092353

Changhyun Lim, James McKendry, Matthew Lees, Philip J Atherton, Nicholas A Burd, Andrew M Holwerda, Luc J C van Loon, Chris McGlory, Cameron J Mitchell, Kenneth Smith, Daniel J Wilkinson, Tanner Stokes, Stuart M Phillips

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Abstract

Understanding the turnover of proteins in tissues gives information as to how external stimuli result in phenotypic change. Nowhere is such phenotypic change more conspicuous than skeletal muscle, which can be effectively remodelled by increased loading, ageing and unloading (disuse), all of which are subject to modification by nutrition and other environmental stimuli. The understanding of muscle proteome remodelling has undergone a renaissance recently with the reintroduction of deuterated water (D₂O) and its ingestion to label amino acids and measure their incorporation into proteins. However, there is confusion around the use of the deuterated water methodology and the interpretation of the data it provides. Here, we provide a short review of some of the more salient features of the method and clarify some of the confusion around the method of deuterated water methods and its use in humans and how the interpretation of the data is in contrast to that of rodents.

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来源期刊

Experimental Physiology 医学-生理学

CiteScore

5.10

自引率

3.70%

发文量

262

审稿时长

1 months

期刊介绍： Experimental Physiology publishes research papers that report novel insights into homeostatic and adaptive responses in health, as well as those that further our understanding of pathophysiological mechanisms in disease. We encourage papers that embrace the journal’s orientation of translation and integration, including studies of the adaptive responses to exercise, acute and chronic environmental stressors, growth and aging, and diseases where integrative homeostatic mechanisms play a key role in the response to and evolution of the disease process. Examples of such diseases include hypertension, heart failure, hypoxic lung disease, endocrine and neurological disorders. We are also keen to publish research that has a translational aspect or clinical application. Comparative physiology work that can be applied to aid the understanding human physiology is also encouraged. Manuscripts that report the use of bioinformatic, genomic, molecular, proteomic and cellular techniques to provide novel insights into integrative physiological and pathophysiological mechanisms are welcomed.