Increasing Burden of Antibiotic Resistance in India Due to Co-existence of Multiple Classes of Carbapenem and Different Aminoglycoside Resistance Genes in Clinical Isolates of Acinetobacter baumannii.

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Current Microbiology Pub Date : 2025-02-06 DOI:10.1007/s00284-025-04097-1

Ashutosh Kumar Amar, Lipsa Panda, Karavali Prasad, Ajit Ramesh Sawant, Meerabai Manoharan, Jagdish Menon, Sujatha Sistla, K Prashanth

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Abstract

Acinetobacter baumannii is a significant pathogen responsible for resistant recalcitrant infections and outbreaks in hospitals worldwide, which is of much concern. The main goal of the present study is to determine the levels of antibiotic resistance in recent clinical isolates of Acinetobacter baumannii and to investigate the presence of diverse resistance determinants among these isolates and also to correlate these findings. A total of 133 A. baumannii clinical isolates from hospitalized patients were investigated for antibiotic resistance levels by determining their MIC by microbroth dilution method. The presence of β-lactamase-encoding genes (bla_PER-1, bla_NDM-1, bla_IMP-1, bla_ADC, bla_OXA51, bla_OXA23, bla_OXA58 and bla_OXA24), aminoglycoside resistance-related genes (armA, aph3(III), aac3(I), aac6, ant3, and aph3(I) and genes associated with biofilm formation were checked by amplifying them by PCR using gene specific primers. The genotyping of isolates was carried out by REP-PCR. Coexistence of diverse intrinsic and acquired carbapenem-hydrolyzing β-lactamases (CHDLs) genes was observed in the isolates: Ambler class A: bla_PER-1 (50.37%), Class B: bla_NDM-1 (63.90%), bla_IMP-1 (76%), Class C: bla_ADC (75.93) and class D: bla_OXA-23 (91.7%), bla_OXA-51 (97.74%). A significant difference (P < 0.05) was observed in the prevalence of bla_OXA-23 among the isolates obtained from ICU (n = 52) patients and with that of non-ICU (n = 81) patients. MIC results revealed 105 (79%) isolates were extremely drug-resistant (XDR) while 28 (21%) isolates were multi-drug-resistant. All the isolates were susceptible only to colistin. Rampant emergence of XDR isolates and co-existence of multiple CHDLs and different aminoglycoside-modifying enzymes genes in A. baumannii isolates in this study points out the increasing burden of antibiotic resistance in our region.

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来源期刊

Current Microbiology 生物-微生物学

CiteScore

4.80

自引率

3.80%

发文量

380

审稿时长

2.5 months

期刊介绍： Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.